扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
RabbitAnti-Fas Ligand Rabbit pAb  antibody (bs-0216R)
~~~促销,代码KXJ230206~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0216R
英文名称 Fas Ligand Rabbit pAb
中文名称 FasL(CD178)抗体
别    名 Tumor necrosis factor ligand superfamily member 6; FASLG; APO1L; Apoptosis(APO 1) antigen ligand 1; Fas-L; Apoptosis antigen ligand 1; APTL; CD178; CD-178; CD 178; CD95L; CD95 ligand; CD95-L; CD95L protein; FAS antigen ligand; Fas L; FASL; Fas ligand(TNF superfamily member 6); Generalized lymphoproliferative disease; TNF superfamily member 6; TNFSF6; TNFL6_HUMAN; Apoptosis antigen ligand; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; FASLG.  
Specific References  (17)     |     bs-0216R has been referenced in 17 publications.
111 [IF=5.699] Janos L. Tanyi. et al. Personalized cancer vaccine strategy elicits polyfunctional T cells and demonstrates clinical benefits in ovarian cancer. Npj Vaccines. 2021 Mar;6(1):1-14  IHC ;  Mouse.  222
111 [IF=5.23] Lv, Yanhong, et al. "Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma." Scientific Reports 7 (2017): 40967.  WB ;  Mouse.  222
111 [IF=4.38] Lai, Ting-Yu. et al. Different methods of detaching adherent cells and their effects on the cell surface expression of Fas receptor and Fas ligand. SCI REP-UK. Sci Rep-Uk. 2022 Apr;12(1):1-8  WB,IF ;  Mouse.  222
111 [IF=4.319] Le Zhang. et al. Dioscin Regulating Bone Marrow Apoptosis in Aplastic Anemia. DRUG DES DEV THER. 2022 Sep;16:3041-3053  WB ;  Mouse.  222
111 [IF=3.53] Song, Xiufang, et al. "Polychlorinated biphenyl quinone metabolite promotes p53-dependent DNA damage checkpoint activation, S-phase cycle arrest and extrinsic apoptosis in human liver hepatocellular carcinoma HepG2 Cells."Chemical Research in Toxicology (2015).  WB ;  Human.  222
111 [IF=2.87] Shi, Yuqin, et al. "β‐benzene hexachloride induces apoptosis of rat sertoli cells through generation of reactive oxygen species and activation of JNKs and FasL." Environmental toxicology 26.2 (2011): 124-135.  WB ;  Rat.  222
111 [IF=2.868] Jimbo H et al. Fas‐FasL interaction in cytotoxic T cell‐mediated vitiligo: The role of lesional expression of tumor necrosis factor‐α and interferon‐γ in Fas‐mediated melanocyte … Exp Dermatol. 2019 Nov 1.  IHC-P ;  Human.  222
111 [IF=2.85] Qi, Suqin, et al. "BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38 MAPK pathways." Reproductive Toxicology 50 (2014): 108-116.  WB ;  Rat.  222
111 [IF=2.766] Zhang et al. Cytochrome P450 3A1 mediates 2,2',4,4'-tetrabromodiphenyl ether-induced reduction of spermatogenesis in adult rats. (2013) PLoS.One. 8:e66301  WB ;  Rat.  222
111 [IF=2.67] Wang, Yi, et al. "Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar Sertoli cells through the regulation of pro‐inflammatory cytokines and miR‐187." Molecular Reproduction and Development (2015).  WB ;  
111 [IF=2.136] Liu S et al. PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology. 2019 Jun;131:89-95.  WB ;  porcine.  222
111 [IF=2.13] Shi, Yuqin, et al. "p, p′-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway." Journal of Biomedicine and Biotechnology (2009).  Rat.  222
111 [IF=1.918] Jisheng Wang. et al. Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway. Med Sci Monitor. 2021; 27: e928232-1–e928232-12  WB ;  Mouse.  222
111 [IF=1.77] Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek??s disease virus infection in the bursa of Fabricius."Research in veterinary science (2012).q  WB ;  Chicken.  222
111 [IF=1.664] Wang Z et al. β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells.Oncol Lett. 2018 Oct;16(4):4519-4525.  WB ;  Human.  222
111 [IF=1.487] Yuefeng Gaoet al. Vitamin E promotes ovine Sertoli cell proliferation by regulation of genes associated with cell division and the cell cycle. Anim Biotechnol . 2020 Jul 2;1-9.  IF ;  sheep.  222
111 [IF=1.48] Wang, Shuhua, Qingqing Tian, and Fang An. "Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells." Oncology Letters (2016).  WB ;  Human.  222
研究领域 肿瘤  细胞生物  免疫学  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Cow, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 31 kDa
检测分子量 38-42
细胞定位 细胞核 细胞膜 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Fas Ligand: 196-281/281 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Function:
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

Subunit:
Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Secreted. Cytoplasmic vesicle lumen. Lysosome lumen. Note=May be released into the extracellular fluid, probably by cleavage form the cell surface. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination.

Post-translational modifications:
N-glycosylated.
The soluble form derives from the membrane form by proteolytic processing.
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
Monoubiquitinated.

DISEASE:
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

Similarity:
Belongs to the tumor necrosis factor family.

SWISS:
P48023

Gene ID:
356

Database links:

Entrez Gene: 356 Human

Entrez Gene: 14103 Mouse

Entrez Gene: 25385 Rat

Omim: 134638 Human

SwissProt: P48023 Human

SwissProt: P41047 Mouse

SwissProt: P36940 Rat

Unigene: 2007 Human

Unigene: 3355 Mouse

Unigene: 9725 Rat



产品图片
Sample: Lane 1: Bone (Mouse) Lysate at 40 ug Lane 2: Bone (Rat) Lysate at 40 ug Primary: Anti-Fas Ligand (bs-0216R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD
Sample: Bone (mouse) Lysate at 40 ug Primary: Anti- Fas ligand (bs-0216R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 46 kD
Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Fas Ligand (bs-0216R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min;
Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal g
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat se
Tissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FasL Poly
Blank control: Mouse Kidney (blue). Primary Antibody:Rabbit Anti-phospho-Fas Ligand antibody (bs-0216R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondar
Blank control: mouse thymouses(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.
版权所有 2004-2023 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    认证编号: 00122Q31509R1M/1100
京ICP备05066980号-1         京公网安备110107000727号