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RabbitAnti-GRP78 Rabbit pAb  antibody (bs-1219R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
50ul/1180.00元
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100ul/1980.00元
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200ul/2800.00元
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产品编号 bs-1219R
英文名称 GRP78 Rabbit pAb
中文名称 葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体
别    名 BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.  
Specific References  (34)     |     bs-1219R has been referenced in 34 publications.
111 [IF=7.675] Reziyamu Wufuer. et al. Distinct Roles of Nrf1 and Nrf2 in Monitoring the Reductive Stress Response to Dithiothreitol (DTT). ANTIOXIDANTS-BASEL. 2022 Aug;11(8):1535  WB ;  Human.  222
111 [IF=6.208] Yu Wu. et al. Geraniol-Mediated Suppression of Endoplasmic Reticulum Stress Protects against Cerebral Ischemia–Reperfusion Injury via the PERK-ATF4-CHOP Pathway. INT J MOL SCI. 2023 Jan;24(1):544  WB ;  Rat.  222
111 [IF=5.96] Xia, Dan, et al. "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress." Cell Death and Disease 8.7 (2017).  WB ;  Human.  222
111 [IF=5.878] Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. 2021 Dec 02  WB ;  Mouse ,Human.  222
111 [IF=5.391] Zhou L et al. KLF15-activating Twist2 ameliorated hepatic steatosis by inhibiting inflammation and improving mitochondrial dysfunction via NF-κB-FGF21 or SREBP1c-FGF21 pathway. FASEB J. 2019 Oct 24:fj201901347RR.  WB ;  Mouse&Human.  222
111 [IF=5.19] Peng Junjun. et al. Mitochondria-associated endoplasmic reticulum membranes participate mitochondrial dysfunction and endoplasmic reticulum stress caused by copper in duck kidney. ENVIRON SCI POLLUT R. 2023 May;:1-12  WB ;  Duck.  222
111 [IF=4.868] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742.  WB ;  Mouse.  222
111 [IF=4.557] Zhao,et al.Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis.(2018) Immunology and Cell Biology. 96:401-412.  IF(IHC-F) ;  Mouse.  222
111 [IF=4.52] Zhu YP et al. Unification of Opposites between Two Antioxidant Transcription Factors Nrf1 and Nrf2 in Mediating Distinct Cellular Responses to the Endoplasmic Reticulum Stressor Tunicamycin. Antioxidants (Basel). 2019 Dec 19;9(1). pii: E4.  WB ;  Human.  222
111 [IF=4.302] Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2.  IHC-P ;  Human.  222
111 [IF=4.014] Xueping Chen. et al. Baicalin ameliorates Mycoplasma gallisepticum-induced inflammatory injury via inhibiting STIM1-regulated ceramide accumulation in DF-1 cells. POULTRY SCIENCE. 2023 Mar;:102687  WB ;  Chicken.  222
111 [IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.  WB ;  Human.  222
111 [IF=3.827] Yuan, Naihan. et al. Replication of standard bovine viral diarrhea strain OregonC24Va induces endoplasmic reticulum stress–mediated apoptosis of bovine trophoblast cells. CELL STRESS CHAPERON. 2022 Nov;:1-12  WB ;  Bovine.  222
111 [IF=3.642] Emin Kaymak. et al. Thymoquinone has a neuroprotective effect against inflammation, oxidative stress, and endoplasmic reticulum stress in the brain cortex, medulla, and hippocampus due to doxorubicin. 2021 Aug 15  IHC ;  Rat.  222
111 [IF=3.606] Xuejiao Zhou. et al. The novel ALK inhibitor ZX‐29 induces apoptosis through inhibiting ALK and inducing ROS‐mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02  WB ;  Human.  222
111 [IF=3.606] Derya Karabulut. et al. Thymoquinone attenuates doxorubicin‐cardiotoxicity in rats. J Biochem Mol Toxic. 2021 Jan;35(1):e22618  IHC ;  Rat.  222
111 [IF=3.37] Du, Shaoqing, et al. "SelK is a novel ER stress-regulated protein and protects HepG2 cells from ER stress agent-induced apoptosis." Archives of biochemistry and biophysics 502.2 (2010): 137-143.  WB ;  Human.  222
111 [IF=3.28] Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13.  WB ;  Mouse.  222
111 [IF=3.219] Yuting Li. et al. Evodiamine suppresses the progression of non-small cell lung carcinoma via endoplasmic reticulum stress-mediated apoptosis pathway in vivo and in vitro:. INT J IMMUNOPATH PH. Int J Immunopath Ph. 2022;():  WB ;  Human,Mouse.  222
111 [IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.  WB ;  Mouse.  222
111 [IF=3.046] Yong-yi Dai. et al. Effects of exogenous vitamin D on endoplasmic reticulum stress-related factors in the vitamin D deficiency rats with cerebral ischemia. Neurosci Lett. 2021 Jul;:136115  WB ;  Rat.  222
111 [IF=2.776] Su M et al.Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress. (2019) Environ Toxicol Pharmacol.65:60-65.  WB ;  Mouse.  222
111 [IF=2.776] He Q et al. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ Toxicol Pharmacol. 2018 Oct;63:6-15.  WB ;  Mouse.  222
111 [IF=2.77] Zuo Y et al. Preoperative vitamin-rich carbohydrate loading alleviates postoperative cognitive dysfunction in aged rats. Behav Brain Res. 2019 Nov 5;373:112107.  WB ;  Rat.  222
111 [IF=2.71] Wei, W, et al. "Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion." Environmental Toxicology 28 (2013):  Human.  222
111 [IF=2.611] Ozturk, E.. et al. Evaluation by different mechanisms of the protective effects of vitamin B12 on methotrexate nephrotoxicity. 2021 Oct 16  IHC ;  Rat.  222
111 [IF=2.147] Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951  IF ;  Human.  222
111 [IF=1.954] Jiang H et al.Strontium Alleviates Endoplasmic Reticulum Stress in a Nonalcoholic Fatty Liver Disease Model.(2018)J Med Food. Nov 17.  IHC ;  Rat.  222
111 [IF=1.851] Lan J et al. Endoplasmic reticulum stress induces liver cells apoptosis after brain death by suppressing the phosphorylation of protein phosphatase 2A. Molecular Medicine Reports 21.2 (2020): 567-574.  WB ;  Rat.  222
111 [IF=1.834] Emin Kaymak. et al. Thymoquinone alleviates doxorubicin induced acute kidney injury by decreasing endoplasmic reticulum stress, inflammation and apoptosis. BIOTECH HISTOCHEM. 2022 Aug 22  IHC ;  Mouse.  222
研究领域 肿瘤  细胞生物  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 78 kDa
检测分子量
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GRP78: 251-360/654 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Function:
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the heat shock protein 70 family.

SWISS:
P11021

Gene ID:
3309

Database links:

Entrez Gene: 3309 Human

Entrez Gene: 14828 Mouse

Entrez Gene: 25617 Rat

Omim: 138120 Human

SwissProt: P11021 Human

SwissProt: P20029 Mouse

SwissProt: P06761 Rat

Unigene: 605502 Human

Unigene: 743241 Human

Unigene: 330160 Mouse

Unigene: 470180 Mouse

Unigene: 11088 Rat



葡萄糖调节蛋白78是热休克蛋白70家族的成员之一，又称：热休克蛋白70蛋白5,作为一种分子伴侣在蛋白质的折叠和转运过程及内质网应激反应中发挥重要作用.它在多种肿瘤组织中呈高表达.
产品图片
Sample: Lane 1: Spleen (Rat) Lysate at 40 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Lane 3: U-87MG (Human) Cell Lysate at 30 ug Lane 4: Hela (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug Lane 7: A431 (Human) Cell Lysate at 30 ug Primary: Anti-GRP78 (bs-1219R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 74 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(bs-1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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