| 产品编号 | bs-1002R |
| 英文名称 | MGMT Rabbit pAb |
| 中文名称 | O6甲基鸟嘌呤DNA甲基转移酶抗体 |
| 别 名 | 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT: O-6 methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6 methylguanine DNA alkyltransferase; O-6-methylguanine-DNA methyltransferase; O6 methylguanine DNA methyltransferase; MGMT_HUMAN. |
 |
Specific References (3) | bs-1002R has been referenced in 3 publications.
111
[IF=3.61] Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014). WB ; Human. 222
111
[IF=2.98] Li et al. Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study. (2018) Oncol.Rep. 39:1609-1618 IHC,WB ; Hamsters. 222
111
[IF=2.886] Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341–8354 ICC ; Rat, Human. 222
|
| 研究领域 | 肿瘤 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGMT: 101-207/207 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients. Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Subcellular Location: Nucleus. Similarity: Belongs to the MGMT family. SWISS: P16455 Gene ID: 4255 Database links: Entrez Gene: 4255 Human Omim: 156569 Human SwissProt: P16455 Human Unigene: 501522 Human O-6-methylguanine-DNA methyltransferase(MGMT)O-6-甲基鸟嘌呤-DNA-甲基转移酶:是广泛存在于细菌到哺乳动物机体中的一种独特DNA修复酶，MGMT是一种DNA修复酶，是突变和癌变的抑制因子。它对烷化剂导致的DNA损伤有修复作用。 MGMT是突变和癌基因的抑制因子。研究表明MGMT水平与恶性肿瘤的发生、进展、浸润、预后及治疗有密切关系。细胞中MGMT水平与其所能耐受的DNA损伤密切相关。一般来讲，低水平表达MGMT的肿瘤可以应用烷化剂药物治疗；反之则为耐药。在正常组织中，肝脏高表达；脑组织和骨髓低水平表达。 |
| 产品图片 |
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
