| 产品编号 | bs-1045R |
| 英文名称 | CCL3 Rabbit pAb |
| 中文名称 | 巨噬细胞炎症因子1α抗体 |
| 别 名 | G0S19-1; LD78; LD78ALPHA; MIP-1-alpha; MIP1A; SCI; SCYA3; MIP-1alpha; MIP1-(a); MIP1-alpha; MIP-1a; CCL3_HUMAN; CCL3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha (MIP-1-alpha); PAT 464.1; SIS-beta; Small-inducible cytokin MIP-1α; MIP-1-α; MIP 1 α; MIP1α; MIP1-α. |
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Specific References (1) | bs-1045R has been referenced in 1 publications.
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[IF=4.4] Lee et al. Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis. (2014) Front.Pharmaco. 5:140 Human,Mouse. 222
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| 研究领域 | 肿瘤 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8 kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MIP-1 alpha: 41-92/92 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated. Function: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Subunit: Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69). Subcellular Location: Secreted. Post-translational modifications: N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. Similarity: Belongs to the intercrine beta (chemokine CC) family. SWISS: P10147 Gene ID: 6348 Database links: Entrez Gene: 6348 Human Entrez Gene: 20302 Mouse Omim: 182283 Human SwissProt: P10147 Human SwissProt: P10855 Mouse Unigene: 514107 Human Unigene: 1282 Mouse Unigene: 10139 Rat 巨噬细胞炎症蛋白1α（MIP-1α）是CC趋化因子中的一种。主要用于炎症、肿瘤方面的研究。 |
| 产品图片 |
Tissue/cell: rat exfoliated cells; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MIP-1 Alpha/CCL3 Polyclonal Antibody, Unconjugated(bs-1045R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
