| 产品编号 | bs-0190R |
| 英文名称 | PACAP-27/38 Rabbit pAb |
| 中文名称 | 腺苷酸环化酶激活肽-27/38抗体 |
| 别 名 | Adenylate Cyclase Activating Polypeptide 1(Pituitary); Prepro-PACAP; PACAP; Pituitary Adenylate Cyclase Activating Polypeptide; PRP-48; PACAP27; PACAP38; ADCYAP1; PACA_HUMAN; Pituitary Adenylate Cyclase-Activating Polypeptide; Adenylate Cyclase Activating Polypeptide 1; PRP48; PACAP-27; PACAP-38; |
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Specific References (4) | bs-0190R has been referenced in 4 publications.
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[IF=8.739] Hailou Zhang. et al. Synergistic effects of two natural compounds of iridoids on rapid antidepressant action by up-regulating hippocampal PACAP signaling. 2022 Mar 31 WB,IF ; Mouse. 222
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[IF=4.755] Han Xuke. et al. Effects of electroacupuncture on bladder dysfunction and the expression of PACAP38 in a diabetic rat model. FRONT PHYSIOL. 2022 Dec;13:2751 WB ; Rat. 222
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[IF=3.188] Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398 IHC ; Rat. 222
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[IF=2.276] Lu L et al. Beneficial Effects of Crocin against Depression via Pituitary Adenylate Cyclase-Activating Polypeptide. Biomed Res Int. 2020 Jun 24;2020:3903125. WB ; Rat. 222
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| 研究领域 | 细胞生物 神经生物学 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Rat, (predicted: Mouse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACAP-27/38: 132-169/176 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013] Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion. Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells. Subunit: Interacts with ADCYAP1R1 (via N-terminal extracellular domain). Subcellular Location: Extracellular region or secreted Similarity: Belongs to the glucagon family. SWISS: P18509 Gene ID: 116 Database links: Entrez Gene: 116 Human Entrez Gene: 11516 Mouse Omim: 102980 Human SwissProt: P18509 Human SwissProt: O70176 Mouse Unigene: 531719 Human Unigene: 727476 Human Unigene: 3407 Mouse Unigene: 37400 Rat 腺苷酸环化酶激活肽是一种可激活垂体腺苷酸环化酶的多肽，有PACAP-27和 PACAP-38两种形式，他们共同具有N端27个氨基酸，属于PACAP/glucagon超级家族。在中枢神经系统分布广泛，其中以下丘脑含量最高。 |
| 产品图片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- PACAP-27/38 (bs-0190R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 20 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
