| 产品编号 | bs-1130R |
| 英文名称 | Gemin 2 Rabbit pAb |
| 中文名称 | 运动神经元存活蛋白结合蛋白1抗体 |
| 别 名 | Component of gems 2; GEMI2_HUMAN; Gemin-2; gemin2; SIP 1; SIP-1; SIP1; SIP1 delta; SMN interacting protein 1; SMN interacting protein 1 delta; SMN-interacting protein 1; Survival interacting protein 1; Survival of motor neuron protein interacting protein 1; Survival of motor neuron protein-interacting protein 1. survival of motor neuron protein interacting protein 1; SMN protein interacting protein 1; KIAA0569; SIP 1; SIP1; SMADIP 1; SMADIP1. |
| 研究领域 | 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SIP1: 51-150/280 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Smad Interacting Protein 1 (SIP1) is observed in neural crest derived cells (peripheric nervous system, enteric nervous system, facial neurectoderm and cranial nerve ganglia), central nervous system, genital tubercle, muscles and kidneys in the developing human. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 can be induced by TGF. SIP1 plays a crucial role in normal embryonic development of neural structures and the neural crest. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR). Patients with SIP1 mutations show mental retardation, delayed motor development, epilepsy, microcephaly, distinct facial features and/or congenital heart disease. SIP1 is also involved in the regulation of epithelial to mesenchymal transition, an important process in tumor progression. Function: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Subunit: Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN5. Subcellular Location: Nucleus, gem. Cytoplasm. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. Similarity: Belongs to the gemin-2 family. SWISS: O14893 Gene ID: 8487 Database links: Entrez Gene: 8487 Human Entrez Gene: 66603 Mouse Omim: 602595 Human SwissProt: O14893 Human SwissProt: Q9CQQ4 Mouse SwissProt: O42260 Xenopus laevis Unigene: 652307 Human Unigene: 35353 Mouse |
| 产品图片 |
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SIP1 Polyclonal Antibody, Unconjugated(bs-1130R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
