| 产品编号 | bs-1034R |
| 英文名称 | SP-B/Surfactant Protein B Rabbit pAb |
| 中文名称 | 肺表面活性蛋白B抗体 |
| 别 名 | 18 kDa pulmonary surfactant protein; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant apoprotein PSP B; Pulmonary surfactant associated protein B 18kD; Pulmonary surfactant associated protein B; Pulmonary surfactant associated prot |
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Specific References (1) | bs-1034R has been referenced in 1 publications.
111
[IF=1.19] Kanie et al. Suppressive effects of the expectorant drug ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats. (2017) J.Toxicol.Pathol. 30:153-159 IHC-P ; Rat. 222
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| 研究领域 | 肿瘤 心血管 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Mouse, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse SP-B: 291-377/377 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.Homodimer; disulfide-linked. Secreted, extracellular space. Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted, extracellular space, surface film. Similarity: Contains 1 saposin A-type domain. Contains 3 saposin B-type domains. SWISS: P50405 Gene ID: 20388 Database links: Entrez Gene: 6439 Human Entrez Gene: 20388 Mouse Omim: 178640 Human SwissProt: P07988 Human SwissProt: P50405 Mouse Unigene: 512690 Human Unigene: 46033 Mouse 肺泡表面活性物质B（SP-B）也属糖结合蛋白家族,参与肺泡表面活性膜的形成和代谢,除在Ⅱ型细胞中强烈表达外，在细支气管、支气管上皮内也有灶性表达。SP-B是疏水性蛋白，主要效应是促进磷脂吸附和分布到肺泡气-液交界面，促进磷脂单分子层的形成，只有这种单分子层磷脂才能使表面张力降低到最低水平。 |
| 产品图片 |
Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Lung (Rat) Lysate at 40 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-SP-B/Surfactant Protein B (bs-1034R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
