| 产品编号 | bs-0381R |
| 英文名称 | Spectrin alpha chain, non-erythrocytic 1 Rabbit pAb |
| 中文名称 | 血影蛋白A链非红细胞型抗体 |
| 别 名 | alpha-II spectrin; SPTN1_HUMAN; alpha Fodrin; Spectrin-alpha; SPEC; Spectrin, alpha, non-erythrocy tic 1 variant; Spectrin alpha chain, non-erythrocytic 1; alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit; SPTAN1; SPTA2; spectrin, non-erythroid alpha chain; alpha-fodrin; spectrin, non-erythroid alpha subunit; spectrin alpha chain, non-erythrocytic 1; EIEE5; NEAS; Spectrin α II; Spectrin α-II; Spectrin αII; Spectrin-α II. |
| 研究领域 | 心血管 细胞生物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Cow, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 272 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Spectrin alpha chain, non-erythrocytic 1: 30-110/2472 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010] Function: Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Subunit: Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts with isoform 1 of ACP1. Interacts with CALM and EMD. Interacts (via C-terminal spectrin repeats) with TRPC4. Identified in a complex with ACTN4, CASK, IQGAP1, MAGI2, NPHS1 and SPTBN1. Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. Post-translational modifications: Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. DISEASE: Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia. Similarity: Belongs to the spectrin family. Contains 3 EF-hand domains. Contains 1 SH3 domain. Contains 23 spectrin repeats. SWISS: Q13813 Gene ID: 6709 Database links: Gene ID: 6709 Human SwissProt: Q13813 Human 血影蛋白(spectrin)又称收缩蛋白，是红细胞膜骨架的主要成份,参与了细胞骨架结构的形成。但不是红细胞膜蛋白的成份,约占膜提取蛋白的30%。血影蛋白属是类似肌球蛋白的膜的外在性蛋白的一种。Spectrin α是膜里侧结构蛋白质之一，除起支持双层脂质外，还有保持红细胞外形的作用，Spectrin-α占里侧结构蛋白之60—70％。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
