| 产品编号 | bs-1291R |
| 英文名称 | ING1 Rabbit pAb |
| 中文名称 | 生长抑制因子基因1抗体 |
| 别 名 | Inhibitor of growth protein 1; inhibitor of growth gene 1; Growth inhibitor ING 1; Growth inhibitor ING1; Growth inhibitory protein ING 1; Growth inhibitory protein ING1; Homo sapiens growth inhibitor p33ING1(ING1) mRNA, complete cds; ING 1; Inhibitor of growth 1; Inhibitor of growth family member 1; Inhibitor of growth protein 1; p24ING1c; p33 ING1; p33ING1; p33ING1b; p47ING1a; Tumor suppressor ING 1; Tumor suppressor ING1. Inhibitor of growth protein 1; ING1_HUMAN; |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human inhibitor of growth gene 1: 301-422/422 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene. Subcellular Location: Nucleus. Tissue Specificity: Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines. DISEASE: Defects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck. Similarity: Belongs to the ING family. Contains 1 PHD-type zinc finger. SWISS: Q9UK53 Gene ID: 3621 Database links: Entrez Gene: 3621 Human Entrez Gene: 26356 Mouse SwissProt: Q9UK53 Human SwissProt: Q9QXV3 Mouse 肿瘤的发生是一个多基因参与,多步骤发生的复杂过程，抑癌基因的功能失活与癌基因的激活为其主要原因之一。 ING1基因是近期发现的抑癌基因，研究发现，它的过表达可抑制细胞生长、增殖,促进细胞凋亡，它的低表达与肿瘤的发生、发展有关。ING1正常时定位于胞核，发挥其诱导凋亡和调控细胞周期的作用，当其易位于胞质时上述作用逐步减弱或消失，在肿瘤发生时，多位于胞浆。 |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- ING1 (bs-1291R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
