| 产品编号 | bs-1298R |
| 英文名称 | Smac Rabbit pAb |
| 中文名称 | 线粒体促凋亡蛋白抗体 |
| 别 名 | Smac/Diablo; 0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog(Drosophila); Diablo homolog(Drosophila); Diablo homolog; Diablo homolog Drosophila; Diablo homolog mitochondrial; Diablo homolog mitochondrial precursor; DIABLO S; DIABLO S; DIABL |
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Specific References (1) | bs-1298R has been referenced in 1 publications.
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[IF=1.96] Liu, Bao-Heng, et al. "Smac/DIABLO regulates the apoptosis of hypertrophic scar fibroblasts."?International Journal of Molecular Medicine?(2013).
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| 研究领域 | 肿瘤 细胞生物 细胞凋亡 新陈代谢 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Smac: 131-239/239 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues. Function: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Subunit: Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Subcellular Location: Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis. Tissue Specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. Post-translational modifications: Ubiquitinated by BIRC7/livin. DISEASE: Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry. SWISS: Q9NR28 Gene ID: 56616 Database links: Entrez Gene: 56616 Human Entrez Gene: 66593 Mouse Omim: 605219 Human SwissProt: Q9NR28 Human SwissProt: Q9JIQ3 Mouse Unigene: 169611 Human Unigene: 46716 Mouse Smac（second mitochondria-derived activator of caspase）又称DIABLO（direct IAP binding protein），是一种线粒体蛋白，也是重要的促细胞凋亡蛋白之一，能够促进细胞凋亡，当杀死不正常细胞的机制启动后，线粒体释放出Smac蛋白促使受损或不正常细胞凋亡的生化过程就会出现，Smac在诱导肿瘤细胞凋亡，增加肿瘤细胞的化疗敏感性及增强免疫力等方面有一定的意义和应用前景。Smac是第二个线粒体来源的胱氨酸酶激活剂，它通过拮抗或中和一种或几种凋亡抑制蛋白（IAP）家族成员而发挥其促凋亡作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
