| 产品编号 | bs-1299R |
| 英文名称 | Parvalbumin Rabbit pAb |
| 中文名称 | 细小清蛋白抗体 |
| 别 名 | D22S749; MGC116759; Parvalbumin alpha; PRVA_HUMAN; PVALB. |
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Specific References (2) | bs-1299R has been referenced in 2 publications.
111
[IF=2.21] Qi et al. Interaction of basolateral amygdala, ventral hippocampus and medial prefrontal cortex regulates the consolidation and extinction of social fear. (2018) Behav.Brain.Funct. 14:7 other ; Mouse. 222
111
[IF=1.68] Chen J et al. Morphology of Inner Retina in Rhesus Monkeys of Various Ages: A Comparative Study.J Ophthalmol. 2019 Mar 3;2019:7089342. IF ; Monkey. 222
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| 研究领域 | 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Parvalbumin: 7-110/110 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Belongs to the parvalbumin family. Contains 2 EF-hand domains. This protein binds two calcium ions. Function: In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Similarity: Belongs to the parvalbumin family. Contains 2 EF-hand domains. SWISS: P20472 Gene ID: 5816 Database links: Entrez Gene: 396459 Chicken Entrez Gene: 396531 Chicken Entrez Gene: 5816 Human Entrez Gene: 19293 Mouse Omim: 168890 Human SwissProt: P20472 Human SwissProt: P32848 Mouse Unigene: 295449 Human Unigene: 2766 Mouse Unigene: 2005 Rat (Parvalbumin, PV)细小白蛋白属于钙结合蛋白家族,是一种细胞内可溶的小结合蛋白，过去曾认为，仅出现在哺乳动物某些肌纤维内,它与Ca2+有高度亲合力,与钙调蛋白竞争自由Ca2+,故可阻止肌浆内的Ca2+增高，可抗衡细胞内钙过量的发生。 |
| 产品图片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-Parvalbumin (bs-1299R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 10 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
