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RabbitAnti-ZO-1/TJP1 Rabbit pAb  antibody (bs-1329R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
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产品编号 bs-1329R
英文名称 ZO-1/TJP1 Rabbit pAb
中文名称 胞质紧密粘连蛋白1/闭锁小带蛋白1抗体
别    名 ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein;  
Specific References  (86)     |     bs-1329R has been referenced in 86 publications.
111 [IF=20.722] Yuting Qin. et al. Colonic mucus-accumulating tungsten oxide nanoparticles improve the colitis therapy by targeting Enterobacteriaceae. Nano Today. 2021 Aug;39:101234  IF ;  Mouse.  222
111 [IF=13.903] Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5.  ICF ;  Mouse.  222
111 [IF=11.061] Narendra K. Singh. et al. Coaxial cell printing of a human glomerular model in vitro of the glomerular filtration barrier and its pathophysiology. BIOFABRICATION. 2022 Dec;:  IF ;  Human.  222
111 [IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119  WB ;  Mouse.  222
111 [IF=9.933] Hohyeon Han. et al. A Bioprinted Tubular Intestine Model Using a Colon-Specific Extracellular Matrix Bioink. 2021 Nov 16  IF ;  Pig.  222
111 [IF=9.229] Xiao Wang. et al. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX  IHC ;  Mouse.  222
111 [IF=8.856] Zhangcai Liu. et al. A Pathomimetic Colorectal Tumor-on-a-Chip for In Vitro Reconstitution of the Primary Colorectal Cancer. ADV MATER TECHNOL-US. 2023 May;:2200758  ICC ;  Human.  222
111 [IF=8.025] Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113  WB ;  Mouse.  222
111 [IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188  WB ;  Mouse.  222
111 [IF=7.546] Jiali Yuan. et al. Effects of metal nanoparticles on tight junction-associated proteins via HIF-1α/miR-29b/MMPs pathway in human epidermal keratinocytes. Part Fibre Toxicol. 2021 Dec;18(1):1-22  IF ;  Human.  222
111 [IF=7.31] Longfei Xiao. et al. Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. OXID MED CELL LONGEV. 2023;2023:1708251  WB,IF ;  Pig,Mouse.  222
111 [IF=6.706] Zhen-Xing Wang. et al. The Barrier-Enhancing Function of Soluble Yam (Dioscorea opposita Thunb.) Polysaccharides in Rat Intestinal Epithelial Cells, as Affected by the Covalent Se Conjugation. NUTRIENTS. 2022 Jan;14(19):3950  WB ;  Rat.  222
111 [IF=6.59] Rongrong Fu. et al. Apigenin remodels the gut microbiota to ameliorate ulcerative colitis.. FRONT NUTR. 2022 Dec;9:1062961-1062961  WB, IHC ;  Mouse.  222
111 [IF=6.543] Deng Liangjun. et al. The Attenuation of Chronic Ulcerative Colitis by (R)-salbutamol in Repeated DSS-Induced Mice. Oxid Med Cell Longev. 2022;2022:9318721  WB,IF ;  Mouse.  222
111 [IF=6.317] Shuo Yang. et al. Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology. FOOD FUNCT. 2022 Oct;:  IHC ;  Mouse.  222
111 [IF=6.317] Zheng Ran. et al. Microbiome–metabolomics analysis reveals the potential effect of verbascoside in alleviating cognitive impairment in db/db mice. FOOD FUNCT. 2023 Mar;:  IHC ;  Mouse.  222
111 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965  WB ;  Duck.  222
111 [IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242  WB ;  Pig.  222
111 [IF=5.988] Yaying You. et al. Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells. FRONT PHARMACOL. 2022; 13: 1052644  WB ;  Human, Mouse.  222
111 [IF=5.878] Chiara Corpetti. et al. Cannabidiol inhibits SARS-Cov-2 spike (S) protein-induced cytotoxicity and inflammation through a PPARγ-dependent TLR4/NLRP3/Caspase-1 signaling suppression in Caco-2 cell line. 2021 Oct 12  WB ;  Human.  222
111 [IF=5.714] Li-Juan You. et al. Schisandrin A ameliorates increased pulmonary capillary endothelial permeability accompanied with sepsis through inhibition of RhoA/ROCK1/MLC pathways. INT IMMUNOPHARMACOL. 2023 May;118:110124  IF,WB ;  Rat,Human.  222
111 [IF=5.572] Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc−/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:113730  WB ;  Mouse.  222
111 [IF=5.546] Yaqi Chang. et al. Glucagon‐like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032  IHC ;  Pig.  222
111 [IF=5.396] Diqi Yang. et al. Baicalin alleviates endometrial inflammatory injury through regulation of tight junction proteins. FOOD FUNCT. 2022 May;:  WB,IF ;  Sheep,Mouse.  222
111 [IF=5.195] Chun Niu. et al. Pulsatilla decoction improves DSS-induced colitis via modulation of fecal-bacteria-related short-chain fatty acids and intestinal barrier integrity. J ETHNOPHARMACOL. 2023 Jan;300:115741  WB ;  Mouse.  222
111 [IF=4.927] Junjie Liu. et al. Phenolic Acids from Fructus Chebulae Immaturus Alleviate Intestinal Ischemia-Reperfusion Injury in Mice through the PPARα/NF-κB Pathway. MOLECULES. 2022 Jan;27(16):5227  IHC ;  Mouse.  222
111 [IF=4.872] Wanfa Dong. et al. Oral delivery of staphylococcal nuclease ameliorates DSS induced ulcerative colitis in mice via degrading intestinal neutrophil extracellular traps. Ecotox Environ Safe. 2021 Jun;215:112161  IHC ;  Mouse.  222
111 [IF=4.651] Masayoshi Ko. et al. Modulation of serotonin in the gut-liver neural axis ameliorates the fatty and fibrotic changes in non-alcoholic fatty liver. Dis Model Mech. 2021 Mar;14(3)  IHC ;  Mouse.  222
111 [IF=4.546] Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against γ-Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816  IHC ;  Mouse.  222
111 [IF=4.546] Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863  WB ;  Pig.  222
研究领域 细胞生物  免疫学  信号转导  细胞粘附分子  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Pig,  (predicted: Mouse, Rat, Chicken, Dog, Cow, Rabbit, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 191 kDa
检测分子量
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ZO-1: 1551-1702/1733 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Function:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Subunit:
Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with CDC42BPB and MYZAP. Interacts (via PDZ domain) with GJA1.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells.

Tissue Specificity:
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Post-translational modifications:
Phosphorylated. Dephosphorylated by PTPRJ.

Similarity:
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain.

SWISS:
Q07157

Gene ID:
7082

Database links:

Entrez Gene: 7082 Human

Entrez Gene: 21872 Mouse

Omim: 601009 Human

SwissProt: Q07157 Human

SwissProt: P39447 Mouse

Unigene: 510833 Human

Unigene: 4342 Mouse



胞质紧密粘连蛋白1（ZO-1）是多结构域蛋白家族膜结合鸟苷酸激酶的家族成员，在紧密连接蛋白的组成成分中起到对组织分化和器官形成方面起较重要的作用。ZO-1在包括肾、胎盘、血脑屏障等许多组织都有不同的表达，可与紧密连接上的很多跨膜蛋白相互作用。也有学者认为：ZO-1的改变与细胞通透性的增加有关。
产品图片
25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 polyclonal antibody, unconjugated (bs-1329R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (bs-1329R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The MCF-7 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (bs-1329R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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