| 产品编号 | bs-1364R |
| 英文名称 | PHLDA1 |
| 中文名称 | T淋巴细胞凋亡相关蛋白TDAG51抗体 |
| 别 名 | Apoptosis associated nuclear protein; Apoptosis-associated nuclear protein; DT1P1B11; MGC131738; PHLA1_HUMAN; PHLDA1; PHRIP; Pleckstrin homology like domain family A member 1; Pleckstrin homology-like domain family A member 1; PQ rich protein; PQ-rich protein; PQR protein; Proline-and glutamine-rich protein; Proline-and histidine-rich protein; T cell death associated gene; T CELL DEATH ASSOCIATED GENE 51; T-cell death-associated gene 51 protein; Tdag; TDAG51. |
| 研究领域 | 细胞生物 细胞凋亡 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Cow, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PHLDA1: 251-360/401 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PHLDA1 is involved in regulation of apoptosis induced by signaling of T cell receptor in murine lympocytes. As any potent regulator with pleiotropic effects related to its overexpression, PHLDA attracts attention in both cell biology research and human tissue pathology. Function: Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation. Subunit: Interacts with RPL14, EIF3S7 and PABPC4. Subcellular Location: Cytoplasm. Cytoplasmic vesicle membrane. Nucleus, nucleolus. Note=Colocalizes with intracellular vesicles. Tissue Specificity: Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level). Similarity: Contains 1 PH domain. SWISS: Q8WV24 Gene ID: 22822 Database links: Entrez Gene: 22822 Human Omim: 605335 Human SwissProt: Q8WV24 Human Unigene: 602085 Human TDAG51参与活化诱导的细胞凋亡，具有促进活化T细胞凋亡的作用,在免疫自稳中发挥重要作用。TDAG51基因在体内各组织中或多或少都有一定的表达，但其功能目前还不清楚，有待于进一步研究。 |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-PHLDA1 (bs-1364R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHLDA1) Polyclonal Antibody, Unconjugated (bs-1364R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PHLDA1/TDAG51 Polyclonal Antibody, Unconjugated(bs-1364R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
