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RabbitAnti-AIF1/Iba1 Rabbit pAb  antibody (bs-1363R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
50ul/1180.00元
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产品编号 bs-1363R
英文名称 AIF1/Iba1 Rabbit pAb
中文名称 离子钙接头蛋白抗体
别    名 AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1.  
Specific References  (34)     |     bs-1363R has been referenced in 34 publications.
111 [IF=32.086] Yuxin Liu. et al. Ferromagnetic flexible electronics for brain-wide selective neural recording. ADV MATER. 2022 Nov;:2208251  IF ;  Rat.  222
111 [IF=10.588] Haiyang Zhang. et al. Co-exposure of chronic stress and alumina nanoparticles aggravates hippocampal microglia pyroptosis by activating cathepsin B/NLRP3 signaling pathway. J HAZARD MATER. 2022 Aug;436:129093  IHC ;  Rat.  222
111 [IF=8.823] Wang Shang. et al. Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimer’s disease in APP/PS1 mouse. ALZHEIMERS RES THER. 2023 Dec;15(1):1-17  WB ;  Mouse.  222
111 [IF=6.208] Guang-Sheng Li. et al. Neurovascular Unit Compensation from Adjacent Level May Contribute to Spontaneous Functional Recovery in Experimental Cervical Spondylotic Myelopathy. INT J MOL SCI. 2023 Jan;24(4):3408  IHC ;  Rat.  222
111 [IF=6.208] Longcong Dong. et al. Shared Genes of PPARG and NOS2 in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation. INT J MOL SCI. 2023 Jan;24(6):5651  IF ;  Mouse.  222
111 [IF=6.023] Tong Wang. et al. Reactive astrocytes induced by 2-chloroethanol modulate microglia polarization through IL-1β, TNF-α, and iNOS upregulation. Food Chem Toxicol. 2021 Sep;:112550  IF ;  rat.  222
111 [IF=5.923] Liang Hu. et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci. 2021 Jan;22(24):13493  WB ;  Pig.  222
111 [IF=5.793] Kong F et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-κB signaling in Alzheimer's disease. J Neuroinflammation . 2020 Oct 15;17(1):305.  IHC ;  Mouse.  222
111 [IF=5.793] Fan’ge Kong. et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-κB signaling in Alzheimer’s disease. J Neuroinflamm. 2020 Dec;17(1):1-15  IHC ;  Mouse.  222
111 [IF=5.23] Hsu, Shih-Tien, et al. "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." Scientific Reports 7 (2017): 42280.  IHC-F ;  Rat.  222
111 [IF=5.195] Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640  IF ;  Rat.  222
111 [IF=4.831] Lushuang Xie. et al. Inflammatory factors and amyloid β-induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538–22549  IHC ;  Rat.  222
111 [IF=4.8] Ko, Chien-Hsin, et al. "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." Scientific reports 7.1 (2017): 17489.  IF(ICC) ;  Rat.  222
111 [IF=4.451] Meilan Xue. et al. Neuroprotective effect of fucoidan by regulating gut-microbiota-brain axis in alcohol withdrawal mice. J Funct Foods. 2021 Nov;86:104726  WB ;  mouse.  222
111 [IF=4.12] Ji et al. TFP5 peptide, derived from CDK5-activating cofactor p35, provides neuroprotection in early-stage of adult ischemic stroke. (2017) Sci.Rep. 7:40013  WB ;  Rat.  222
111 [IF=4.074] Dingkang Xu. et al. Sphingosine‐1‐phosphate receptor 3 is implicated in BBB injury via the CCL2‐CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28  IHC ;  Human.  222
111 [IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653  WB ;  Human.  222
111 [IF=3.82] Fu, Juanli, et al. "The acute exposure of tetrachloro-p-benzoquinone (aka chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning." Toxicology (2017).  IHC-P ;  Mouse.  222
111 [IF=3.61] Qinrui Hu. et al. Neuroprotection of boropinol-B in Cerebral Ischemia-reperfusion Injury by Inhibiting Inflammation and Apoptosis. BRAIN RES. 2022 Oct;:148132  IF ;  Rat.  222
111 [IF=3.548] Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350.  IHF-F ;  Mouse.  222
111 [IF=3.056] Yanan Shen. et al. Phosphatase and Tensin Homolog Deleted on Chromosome Ten Knockdown Attenuates Cognitive Deficits by Inhibiting Microglia-mediated Neuroinflammation in the Hippocampus in a Mouse Model of Perioperative Neurocognitive Disorder. Neuroscience. 2021 Aug;468:199  WB ;  Mouse.  222
111 [IF=3.038] Pengfei Fu. et al. Baicalein Ameliorates Epilepsy Symptoms in a Pilocarpine-Induced Rat Model by Regulation of IGF1R. Neurochem Res. 2020 Dec;45(12):3021-3033  IF ;  Rat.  222
111 [IF=2.866] Wei, Ying. et al. Astrocyte Activation, but not Microglia, Is Associated with the Experimental Mouse Model of Schizophrenia Induced by Chronic Ketamine. J MOL NEUROSCI. 2022 Jul;:1-14  WB ;  Mouse.  222
111 [IF=2.86] Hu. et al. Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway  IF(IHC-P) ;  Mouse.  222
111 [IF=2.86] Hu. et al. Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway  Mouse.  222
111 [IF=2.667] Liao CF et al. Effects of endogenous inflammation signals elicited by nerve growth factor, interferon-γ, and interleukin-4 on peripheral nerve regeneration. J Biol Eng. 2019 Nov 13;13:86.  IHF-F ;  Rat.  222
111 [IF=2.21] Can Sezer. et al. Neuroprotective Effects of Milrinone on Acute Traumatic Brain İnjury. WORLD NEUROSURG. 2022 Nov;:  IHC ;  Mouse.  222
111 [IF=2.14] Ogawa, Takashi, et al. "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague–Dawley rat." Journal of Toxicologic Pathology 0 (2016).  IHC-P ;  Rat.  222
111 [IF=1.797] Ya‐Zhou Lu. et al. The seed oil of Paeonia ludlowii ameliorates Aβ25‐35‐induced Alzheimer's disease in rats. 2021 Mar 30  IHC ;  Rat.  222
111 [IF=1.756] Yue J et al. Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex. Brain Dev. 2019 Sep 5. pii: S0387-7604(19)30240-2.  IHF ;  Human.  222
研究领域 细胞生物  免疫学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 16 kDa
检测分子量
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Iba1: 51-147/147 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.

Function:
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Subunit:
Homodimer (Potential). Monomer. Interacts with LCP1.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Tissue Specificity:
Detected in T-lymphocytes and peripheral blood mononuclear cells.

Similarity:
Contains 2 EF-hand domains.

SWISS:
P55008

Gene ID:
199

Database links:

Entrez Gene: 199 Human

Entrez Gene: 11629 Mouse

Entrez Gene: 29427 Rat

Omim: 601833 Human

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

Unigene: 76364 Human

Unigene: 10747 Mouse

Unigene: 32080 Rat



离子钙接头蛋白主要在小胶质细胞表达.
产品图片
25 ug total protein per lane of various lysates (see on figure) probed with AIF1 (9A3) polyclonal antibody, unconjugated (bs-1363R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
araformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat small Intestine;; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
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