| 产品编号 | bsm-0954M |
| 英文名称 | ET-1(2C4) Mouse mAb |
| 中文名称 | 内皮素-1单克隆抗体 |
| 别 名 | Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1 |
 |
Specific References (2) | bsm-0954M has been referenced in 2 publications.
111
[IF=3.54] Mai et al. Dyssynchronous pacing triggers endothelial-mesenchymal transition through heterogeneity of mechanical stretch in a canine model. (2014) Circ.. 79:201-9 WB ; Canine. 222
111
[IF=1.924] Di Cristofori A et al. Meningioma and Bone Hyperostosis: Expression of Bone Stimulating Factors and Review of the Literature.World Neurosurg. 2018 Jul;115:e774-e781. IHC-P ; Human. 222
|
| 研究领域 | 心血管 神经生物学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 2C4 |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Sheep, Guinea Pig, ) |
| 产品应用 | ELISA=1:5000-20000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 2.5/24 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ET-1(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11): 53-73/202 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Function: Endothelins are endothelium-derived vasoconstrictor peptides. Subcellular Location: Secreted. Tissue Specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Similarity: Belongs to the endothelin/sarafotoxin family. SWISS: P05305 Gene ID: 1906 Database links: Entrez Gene: 1906 Human Entrez Gene: 13614 Mouse Omim: 131240 Human SwissProt: P05305 Human SwissProt: P22387 Mouse Unigene: 511899 Human Unigene: 713645 Human Unigene: 10918 Rat 内皮素(Endothelin-1,ET)是一种强的血管收缩剂，分为ET-1,ET-2,ET-3 三种,ET-1主要在内皮细胞中表达,而ET-2和ET-3则主要在神经系统中表达。ET具有广泛的生物学作用，此抗体是针对内皮素的活性片段（CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11）。 |
| 产品图片 |
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ET-1 Polyclonal Antibody, Unconjugated(bsm-0954M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
