| 产品编号 | bsm-1622M |
| 英文名称 | AFP(A4) Mouse mAb |
| 中文名称 | 甲胎蛋白单克隆抗体(检测) |
| 别 名 | alpha Fetoprotein; alpha-fetoprotein; alpha 1 fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN. |
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Specific References (6) | bsm-1622M has been referenced in 6 publications.
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[IF=6.641] Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019. SERS-based sandwich immunoassay ;
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[IF=6.45] Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014). other ;
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[IF=6.277] Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019. Other ;
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[IF=5.88] Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016). other ;
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[IF=5.83] Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415. other ; Human. 222
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[IF=2.475] Huang Y et al. Ag@Au Core⁻Shell Porous Nanocages with Outstanding SERS Activity for Highly Sensitive SERS Immunoassay. Sensors (Basel). 2019 Mar 31;19(7). pii: E1554. ELISA ;
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| 研究领域 | 肿瘤 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | A4 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 65 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | AFP protein: full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Subunit: Dimeric and trimeric forms have been found in addition to the monomeric form. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized by the fetal liver and yolk sac. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. Database links: Entrez Gene: 174 Human Omim: 104150 Human SwissProt: P02771 Human Unigene: 518808 Human |
| 产品图片 |
Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. bs-43140P) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 20.04 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
