| 产品编号 | bsm-1625M |
| 英文名称 | PSA(Pa3) Mouse mAb |
| 中文名称 | 人前列腺特异性抗原单克隆抗体(包被) |
| 别 名 | antigen, prostate-specific; APS; gamma seminoprotein; gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein 3, (prostate specific antigen); Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; KLK3; KLK3_HUMAN; P 30 antigen; P-30 antigen; P30 antigen; Prostate Specific Antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin. |
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Specific References (4) | bsm-1625M has been referenced in 4 publications.
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[IF=7.243] Fanchun Zeng. et al. Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/β-catenin pathway. GENES DIS. 2022 Jul;: IHC ; Mouse. 222
111
[IF=5.401] Liu, Yingshuai, Lingli Lei, and Zeying Zhang. "An ultrasensitive colorimetric immunoassay based on glucose oxidase catalyzed cascade formation of blue–black iodine–starch complex." Sensors and Actuators B: Chemical (2017).
111
[IF=4.71] Liu, Yingshuai, et al. "A concentration− dependent multicolor conversion strategy for ultrasensitive colorimetric immunoassay with the naked eye." Analytica Chimica Acta (2017). ELISA ; Rabbit. 222
111
[IF=4.04] Zhou, Jie, et al. "Spectrum-based and color-selective electrochemiluminescence immunoassay for determining human prostate specific antigen in near-infrared region." Talanta 165 (2017): 117-121. other ; Human. 222
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| 研究领域 | 肿瘤 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | Pa3 |
| 交叉反应 | Human, |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | ≥1mg/ml |
| 免 疫 原 | Full length native protein purified from human: full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Function: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Subunit: Forms heterodimer with SERPINA5. Subcellular Location: Secreted. Tissue Specificity: Prostate. Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. SWISS: P07288 Gene ID: 354 Database links: Entrez Gene: 354 Human Omim: 176820 Human SwissProt: P07288 Human Unigene: 171995 Human PSA-前列腺特异性抗原为激肽释放酶家族蛋白，为丝氨酸蛋白酶，由前列腺上皮产生，精液中的浓度很高，可使精液凝块水解、液化，功能与男性生育力有关. 此抗体可用于试剂盒包被用抗体。 |
| 产品图片 |
Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PSA(Pa3) Polyclonal Antibody, Unconjugated(bsm-1625M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
