| 产品编号 | bs-1577R |
| 英文名称 | KLF13 Rabbit pAb |
| 中文名称 | 肠道内富含的Kruppel样因子13kt |
| 别 名 | BTEB3; FKLF2; NSLP1; RFLAT-1; RFLAT1; 0610043C13Rik; 9430029L20Rik; FKLF-2; KLF13_HUMAN; KLF13; Basic transcription element-binding protein 3 (BTE-binding protein 3); Novel Sp1-like zinc finger transcription factor 1; RANTES factor of late activated T-lym |
| 研究领域 | 细胞生物 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLF13: 197-249/288 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Kruppel-like factor 13 (KLF13) belongs to a family of transcription factors that contain three classical zinc finger DNA binding domains consisting of a zinc atom tetrahedrally coordinated by two cysteines and two histidines (C2H2 motif). These transcription factors bind to GC rich sequences and related GT and CACCC boxes. KLF13 appears to be an important component of the transcription network required for heart development and cholesterol homeostasis. Function: Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated. Similarity: Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: Q9Y2Y9 Gene ID: 51621 Database links: Entrez Gene: 51621 Human Entrez Gene: 50794 Mouse Omim: 605328 Human SwissProt: Q9Y2Y9 Human SwissProt: Q9JJZ6 Mouse Unigene: 525752 Human Unigene: 240473 Mouse |
| 产品图片 |
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KLF13 Polyclonal Antibody, Unconjugated(bs-1577R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
