| 产品编号 | bs-1863R |
| 英文名称 | DNAJC3 Rabbit pAb |
| 中文名称 | 干扰素诱导蛋白p58ipk抗体 |
| 别 名 | DNAJC3; p58ipk; Protein Kinase Inhibitor P58; DnaJ homolog subfamily C member 3; HP58; Interferon induced, double stranded RNA activated protein kinase inhibitor; P58; p58 ipk; P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; DNJC3_HUMAN. |
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Specific References (1) | bs-1863R has been referenced in 1 publications.
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[IF=4.26] Taguchi, Yoshimitsu, et al. "Sphingosine-1-phosphate phosphatase 2 regulates pancreatic islet β-cell endoplasmic reticulum stress and proliferation." Journal of Biological Chemistry 291.23 (2016): 12029-12038. IHC-P ; Mouse. 222
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| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human p58ipk: 351-450/504 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DNAJC3 may act as an inhibitor of both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2/PKR to catalyze phosphorylation of the EIF2A. It also functions as a Co-chaperone of HSPA8/HSC70 and is involved in the unfolded protein response (UPR) during ER stress. It has been reported that this protein stimulates the ATPase activity of HSPA8/HSC70.DNAJC3 is also involved in ativiral defense - it is activated in response to infection by influenza virus through the dissociation of DNAJB1. DNAJC3 is widely expressed and can be found at high levels in the testis and pancreas (expression in cell lines is variable). Function: Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. Subcellular Location: Endoplasmic reticulum. Tissue Specificity: Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels. Similarity: Contains 1 J domain. Contains 9 TPR repeats. SWISS: Q13217 Gene ID: 5611 Database links: Entrez Gene: 5611 Human Entrez Gene: 100037258 Mouse Omim: 601184 Human SwissProt: Q13217 Human SwissProt: Q91YW3 Mouse Unigene: 59214 Human Unigene: 12616 Mouse Unigene: 162234 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (DNAJC3) Polyclonal Antibody, Unconjugated (bs-1863R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
