| 产品编号 | bs-1599R |
| 英文名称 | Calpain 2 Rabbit pAb |
| 中文名称 | 钙激活的中性蛋白酶2抗体 |
| 别 名 | Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; CALP80; Calpain 2(m/II) large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large subunit; Calpain large polypeptide L2; Calpain M type; Cal |
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Specific References (1) | bs-1599R has been referenced in 1 publications.
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[IF=6.064] Mengni Bao. et al. N-Acetylcysteine, an ROS Inhibitor, Alleviates the Pathophysiology of Hyperthyroidism-Induced Cardiomyopathy via the ROS/Ca2+ Pathway. BIOMOLECULES. 2022 Sep;12(9):1195 WB ; Mouse, Rat. 222
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| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 78 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Calpain 2: 301-400/700 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Subunit: Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Subcellular Location: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. Tissue Specificity: Ubiquitous. Similarity: Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 2 EF-hand domains. Database links: Entrez Gene: 824 Human Entrez Gene: 12334 Mouse Omim: 114230 Human SwissProt: P17655 Human SwissProt: O08529 Mouse Unigene: 350899 Human Unigene: 19306 Mouse Unigene: 6822 Rat 钙蛋白酶2(calpain2)也是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,广泛存在于全身各脏器组织中。钙蛋白酶参与不同细胞过程,包括细胞骨架和细胞膜附着的构型重建、不同的信号转导途径和细胞凋亡等. |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Calpain 2 (bs-1599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Calpain 2(bs-1599R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 90 kD
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calpain 2) Polyclonal Antibody, Unconjugated (bs-1599R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
