| 产品编号 | bs-1684R |
| 英文名称 | SKI Rabbit pAb |
| 中文名称 | C-SKI抗体 |
| 别 名 | C-SKI; v-SKI; Sloan-kettering viral oncogene homolog; Avian sarcoma viral(v ski) oncogene homolog; C oncogene; C ski; Ski oncogene; Ski oncoprotein; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog; v-ski sarcoma viral oncogene homolog; SKI_HUMAN. |
| 研究领域 | 肿瘤 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 80 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C-SKI: 551-650/728 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SKI is a nuclear protein that forms homodimers and heterodimers and binds to DNA to function as transcriptional activators and repressors. The SKI oncoprotein dramatically affects cell growth, differentiation, and/or survival. SKI was shown to act in distinct signaling pathways including those involving nuclear receptors, transforming growth factor beta, and tumor suppressors. SKI inhibits transforming growth factor-beta (TGF-beta) signaling through interaction with Smad proteins. SKI represses Smad-mediated transcriptional activation, probably through its action as a transcriptional co-repressor. SKI also inhibits TGF-beta-induced downregulation of genes such as c-myc. Function: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. Subunit: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. Subcellular Location: Nucleus. Similarity: Belongs to the SKI family. SWISS: P12755 Gene ID: 6497 Database links: Entrez Gene: 6497 Human Entrez Gene: 20481 Mouse Omim: 164780 Human SwissProt: P49140 Chicken SwissProt: P12755 Human SwissProt: Q60698 Mouse Unigene: 656507 Human SKI又称SKI、SKV，经研究发现SKI蛋白是刺激和诱发肿瘤生长导致癌症的关键蛋白质之一，SKI蛋白主要通过阻止TGF-β蛋白发挥作用,当TGF-β蛋白的信号传导被SKI蛋白阻断后，细胞就可能加速增值、生长，最终导致癌症发生。 有研究表明：c-ski可调节多种核因子的转录活性,参与神经系统发育、造血细胞的增殖和分化、肿瘤发生、组织再生等多种生理病理过程，更详细的作用机制还在进一步研究之中。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
