| 产品编号 | bs-1688R |
| 英文名称 | HSPB6 Rabbit pAb |
| 中文名称 | 热休克蛋白-20抗体 |
| 别 名 | HEL55; Hsp20; PPP1R91; Gm479; p20; HSPB6_HUMAN; HSPB6; Heat shock 20 kDa-like protein p20; Heat shock protein family B member 6; Q6NVI3_HUMAN; HSPB6_MOUSE; HSPB6_RAT; Heat shock 20 kDa-like protein p20 (Hsp20); |
| 研究领域 | 肿瘤 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Cow, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 细胞定位 | 细胞核 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSPB6: 6-80/160 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction. Subunit: Homodimer (By similarity). Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the small heat shock protein (HSP20) family. SWISS: Q6NVI3 Gene ID: 126393 Database links: Entrez Gene: 126393 Human Entrez Gene: 243912 Mouse SwissProt: O14558 Human SwissProt: Q6NVI3 Human SwissProt: Q5EBG6 Mouse Unigene: 534538 Human Unigene: 34885 Mouse Unigene: 3201 Rat HSPs是细胞受应激原刺激后诱导产生的一组应激蛋白，与肿瘤发生、增殖及分化有关。按其分子量不同可分为多种类型，每组的HSPs的分布及功能有所不同。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP20) Polyclonal Antibody, Unconjugated (bs-1688R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
