| 产品编号 | bs-1661R |
| 英文名称 | Cytokeratin 6 Rabbit pAb |
| 中文名称 | 细胞角蛋白6抗体 |
| 别 名 | K6E; KRT6E; PPKNEFD; CK-6C; CK-6E; CK6A; CK6C; CK6D; K6A; K6C; K6D; KRT6C; KRT6D; PC3; CK-6B; CK6B; K6B; KRTL1; PC2; PC4; 2310016L08Rik; CK-6A; K6[a]; Krt2-6a; Krt2-6c; MK6a; mK6[a]; Krt2-6b; mK6[b]; K2C6A_HUMAN; KRT6A; Cytokeratin-6A (CK-6A); Cytokeratin |
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Specific References (1) | bs-1661R has been referenced in 1 publications.
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[IF=3.272] Miss Hongfang Meng. et al. Overexpression of hepatocyte growth factor in dental pulp stem cells ameliorates the severity of psoriasis by reducing inflammatory responses. 2021 Jun 22 IHC ; Mouse. 222
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| 研究领域 | 肿瘤 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 检测分子量 | 60 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytokeratin 6: 401-500/564 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin(KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]. Subunit: Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17. Interacts with TCHP. Tissue Specificity: Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. DISEASE: Defects in KRT6A are a cause of pachyonychia congenital type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present. Similarity: Belongs to the intermediate filament family. SWISS: P48668 Gene ID: 3854 Database links: Entrez Gene: 286887 Human Entrez Gene: 3853 Human Entrez Gene: 3854 Human Entrez Gene: 16687 Mouse Entrez Gene: 16688 Mouse Omim: 148041 Human Omim: 148042 Human Omim: 612315 Human SwissProt: P02538 Human SwissProt: P04259 Human SwissProt: P48668 Human SwissProt: P50446 Mouse SwissProt: Q9Z331 Mouse Unigene: 700779 Human Unigene: 709234 Human 结构蛋白（Structural Proteins）细胞角蛋白是一类与结构相关的蛋白家族，其在上皮细胞中形成细胞骨架中间丝。CK6表达于鳞状上皮细胞，特别是增生的鳞状上皮。76%的脑、颈部鳞状细胞癌病例中有CK6的表达。 |
| 产品图片 |
Sample:
Tongue (Mouse) Lysate at 40 ug
Primary: Anti-Cytokeratin 6 (bs-1661R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Blank control（black line）:MCF-7.
Primary Antibody (green line): Rabbit Anti-Cytokeratin 6 antibody (bs-1661R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
