| 产品编号 | bs-1535R |
| 英文名称 | Alkaline Phosphatase, Tissue Non-Specific isozyme Rabbit pAb |
| 中文名称 | 碱性磷酸酶(组织非特异性)抗体 |
| 别 名 | AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. 碱性磷酸酶，组织非特异性同工酶; 组织非特异性碱性磷酸酶; 碱性磷酸酶,组织非特异性; |
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Specific References (4) | bs-1535R has been referenced in 4 publications.
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[IF=5.595] Cao D et al. Hematopoietic stem cells and lineage cells undergo dynamic alterations under microgravity and recovery conditions.FASEB J. 2019 Feb 27:fj201802421RR. FCM ; Mouse. 222
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[IF=4.545] Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235. IHC/IF ; Mouse. 222
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[IF=2.147] Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913 WB ; Dog. 222
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[IF=1.931] Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034 WB ; Human. 222
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| 研究领域 | 细胞生物 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Alkaline Phosphatase, Tissue Non-Specific isozyme: 52-150/524 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Function: This isozyme may play a role in skeletal mineralization. Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Post-translational modifications: Glycosylated. DISEASE: Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510]. Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500]. Similarity: Belongs to the alkaline phosphatase family. SWISS: P05186 Gene ID: 249 Database links: Entrez Gene: 249 Human Entrez Gene: 11647 Mouse Omim: 171760 Human SwissProt: P05186 Human SwissProt: P09242 Mouse Unigene: 75431 Human Unigene: 288186 Mouse Unigene: 82764 Rat ALP广泛分布于人体肝脏、骨骼、肠、肾和胎盘等组织,孕妇、骨折愈合期、骨软化症。佝偻病、骨细胞癌、骨质疏松、肝脓肿、肝结核、肝硬变、白血病、甲状腺机能亢进时，血清碱性磷酸酶亦可升高. |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Alkaline Phosphatase, Tissue Non-Specific isozyme polyclonal antibody, unconjugated (bs-1535R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
