| 产品编号 | bs-1858R |
| 英文名称 | Netrin 1 Rabbit pAb |
| 中文名称 | 轴突导向因子1抗体 |
| 别 名 | Netrin-1; Netrin1; Netrin 1; Ntn 1; NTN 1L; Ntn1; Ntn-1; NTN1L; Unc6. |
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Specific References (5) | bs-1858R has been referenced in 5 publications.
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[IF=4.315] Zongding Wang. et al. Netrin-1 promotes liver regeneration possibly by facilitating vagal nerve repair after partial hepatectomy in mice. Cell Signal. 2022 Mar;91:110227 IHC ; Mouse. 222
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[IF=3.467] Victor H. Guaiquil. et al. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. EXP EYE RES. Exp Eye Res. 2022 Apr;:109054 IF ; Mouse. 222
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[IF=2.766] Chhabra V et al. Hypobaric hypoxia induced renal damage is mediated by altering redox pathway.PLoS One. 2018 Jul 13;13(7):e0195701. ELISA ; Rat. 222
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[IF=1.49] Ko CL et al. Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. High Alt Med Biol. 2019 Jul 22. IHF ; Mouse. 222
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[IF=0.872] Sato Y et al. Expression of Draxin in Lung Carcinomas. (2018) ACTA HISTOCHEMICA ET CYTOCHEMICA, Feb 27;51(1):53-62. IHC ; Human. 222
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| 研究领域 | 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NtN1: 501-604/604 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008] Function: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. Subunit: Binds to its receptors; DCC, DSCAM, UNC5A, UNC5B, UNC5C and probably UNC5D. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Similarity: Contains 3 laminin EGF-like domains. Contains 1 laminin N-terminal domain. Contains 1 NTR domain. SWISS: O95631 Gene ID: 9423 Database links: Entrez Gene: 9423 Human Entrez Gene: 18208 Mouse Omim: 601614 Human SwissProt: O95631 Human SwissProt: O09118 Mouse Unigene: 128002 Human Unigene: 660885 Human Unigene: 39095 Mouse Unigene: 41052 Rat Netrin-1是与层粘连蛋白相关的、高度保守的小分子分泌蛋白家族成员的一种。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Netrin 1) Polyclonal Antibody, Unconjugated (bs-1858R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Netrin 1) Polyclonal Antibody, Unconjugated (bs-1858R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Netrin 1) polyclonal Antibody, Unconjugated (bs-1858R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
