| 产品编号 | bs-1859R |
| 英文名称 | NGB Rabbit pAb |
| 中文名称 | 脑红蛋白/神经血红蛋白/神经球蛋白抗体 |
| 别 名 | neuroglobin; NGB; NGB_MOUSE. |
 |
Specific References (2) | bs-1859R has been referenced in 2 publications.
111
[IF=3.77] Hui-xian Li. et al. PACAP-derived mutant peptide MPAPO protects trigeminal ganglion cell and the retina from hypoxic injury through anti-oxidative stress, anti-apoptosis, and promoting axon regeneration. Bba-Gen Subjects. 2021 Dec;1865:130018 IF ; mouse . 222
111
[IF=1.95] Xiaohua Du. et al. Expression and distribution of neuroglobin and hypoxia-inducible factor-1α in the adult yak telencephalon. 2021 Jun 19 WB ; Yak. 222
|
| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Pig, Cow, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse NGB: 51-151/151 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008] Function: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Subunit: Monomer. Homodimer and homotetramer; disulfide-linked. Subcellular Location: Perikaryon. Tissue Specificity: Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus. Post-translational modifications: A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO. Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity. Similarity: Belongs to the globin family. SWISS: Q9ER97 Gene ID: 64242 Database links: Entrez Gene: 58157 Human Entrez Gene: 64242 Mouse Omim: 605304 Human SwissProt: Q9NPG2 Human SwissProt: Q9ER97 Mouse Unigene: 274363 Human Unigene: 64645 Rat Neuroglobin是一种在神经组织和神经冲动中携氧、转运氧气和供氧的特异性蛋白,并和神经组织微循环有关,神经球蛋白能在缺氧的环境下保护神经元. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (NGB) Polyclonal Antibody, Unconjugated (bs-1859R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
