| 产品编号 | bs-1911R |
| 英文名称 | TGM3 Rabbit pAb |
| 中文名称 | 谷氨酰胺转胺酶3抗体 |
| 别 名 | Transglutaminase 3; E polypeptide protein glutamine gamma glutamyltransferase; MGC126249; MGC126250; Protein glutamine gamma glutamyltransferase E 27 kDa catalytic chain; Protein glutamine gamma glutamyltransferase E 50 kDa noncatalytic chain; Protein glutamine gamma glutamyltransferase E; TG(E); TGase E; TGE; TGM 3; TGM3; Transglutaminase E; Transglutaminase3; TransglutaminaseE; TGM3_HUMAN. |
 |
Specific References (1) | bs-1911R has been referenced in 1 publications.
111
[IF=2.47] Kawabe, Kenji, et al. "Transglutaminases Derived from Astrocytes Accelerate Amyloid β Aggregation." Neurochemical Research: 1-8. Rat. 222
|
| 研究领域 | 神经生物学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25/76 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGM3: 601-693/693 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle. Subunit: Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide. Similarity: Belongs to the transglutaminase superfamily. Transglutaminase family. SWISS: Q08188 Gene ID: 7053 Database links: Entrez Gene: 7053 Human Entrez Gene: 21818 Mouse Omim: 600238 Human SwissProt: Q08188 Human SwissProt: Q08189 Mouse Unigene: 2022 Human Unigene: 2961 Mouse TGM3是转谷氨酰胺酶家族的主要成员之一，是一种钙离子依赖性蛋白酶，主要功能是参与角质化细胞的终末分化过程。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGM3) Polyclonal Antibody, Unconjugated (bs-1911R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
