| 产品编号 | bs-1945R |
| 英文名称 | WNK3 Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸激酶家族成员的基因WNK3抗体 |
| 别 名 | Serine/threonine-protein kinase WNK3; WNK lysine deficient protein kinase 3; Protein kinase with no lysine 3; Protein kinase, lysine-deficient 3; Wnk3; RGD1563131; KIAA1566; PRKWNK 3; PRKWNK-3; PRKWNK3; Protein kinase lysine deficient 3; Protein kinase lysine-deficient 3; Serine/threonine-protein kinase WNK3; WNK 3; WNK3_HUMAN. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Cow, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 198 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNK3: 451-550/1800 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
WNK3 (WNK lysine deficient protein kinase 3) belongs to the serine/threonine protein kinase family. It lacks the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. A conserved lysine in subdomain I is thought to provide this function. WNK3 activates Na-(K)-Cl cotransporters by increasing their phosphorylation and appears to be a crucial component of the kinase/phosphatase signaling pathway that coordinately regulates the Cl– influx and efflux branches of the SLC12A cotransporter family. Subunit: Interacts with WNK1 and WNK4. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3 is kidney-specific. Similarity: BBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. Contains 1 protein kinase domain. SWISS: Q9BYP7 Gene ID: 65267 Database links: Entrez Gene: 65267 Human Omim: 300358 Human SwissProt: Q9BYP7 Human Unigene: 92423 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
