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RabbitAnti-iNOS Rabbit pAb  antibody (bs-2072R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
50ul/1180.00元
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100ul/1980.00元
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200ul/2800.00元
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大包装/询价

产品编号 bs-2072R
英文名称 iNOS Rabbit pAb
中文名称 一氧化氮合成酶-2（诱导型）抗体
别    名 i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A(inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN.  
Specific References  (43)     |     bs-2072R has been referenced in 43 publications.
111 [IF=13.281] Yang Sun. et al. Dual Biosignal-Functional Injectable Microspheres for Remodeling Osteogenic Microenvironment. SMALL. 2022 Apr 14  IHC ;  Rabbit.  222
111 [IF=8.786] Duchenhui Li. et al. The immunomodulatory effect of IL-4 accelerates bone substitute material-mediated osteogenesis in aged rats via NLRP3 inflammasome inhibition. FRONT IMMUNOL. 2023; 14: 1121549  IF ;  Rat.  222
111 [IF=8.579] Tong L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.  IHC ;  Mouse.  222
111 [IF=7.129] Xiao Wang. et al. Salvia miltiorrhiza polysaccharides alleviate florfenicol-induced inflammation and oxidative stress in chick livers by regulating phagosome signaling pathway. ECOTOX ENVIRON SAFE. 2023 Jan;249:114428  IHC ;  Chicken.  222
111 [IF=6.32] Yang,et al.Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells.(2018) Analytical Chemistry. 90:2355-2361.  Other ;  Human.  222
111 [IF=5.988] Yang Liu. et al. Platycodon grandiflorus polysaccharides deeply participate in the anti-chronic bronchitis effects of platycodon grandiflorus decoction, a representative of "the lung and intestine are related". FRONT PHARMACOL. 2022 Sep 7;13:927384  IF ;  Rat.  222
111 [IF=5.62] Derya Kaya. et al. Allopurinol attenuates repeated traumatic brain injury in old rats: A preliminary report. EXP NEUROL. 2022 Nov;357:114196  IHC ;  Rat.  222
111 [IF=5.168] Necla Deger. et al. Thymoquinone regulates nitric oxide synthase enzymes and receptor-interacting serine-threonine kinases in isoproterenol-induced myocardial infarcted rats. CHEM-BIOL INTERACT. 2022 Aug;:110090  IHC ;  Rat.  222
111 [IF=5.076] Vairappan B et al. Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl4-Induced Cirrhotic Mice. Molecular Neurobiology. Oct 30.  WB&IHC ;  Mouse.  222
111 [IF=3.83] Xiong et al. Salvianolic Acid B Restored Impaired Barrier Function via Downregulation of MLCK by microRNA-1 in Rat Colitis Model. (2016) Front.Pharmacol. 7:134  WB ;  rat.  222
111 [IF=3.761] Toru Nishikawa et al. Therapeutic potential for insulin on type 1 diabetes‐associated periodontitis: Analysis of experimental periodontitis in streptozotocin‐induced diabetic rats. J Diabetes Investig. 2020 Nov;11(6):1482-1489.  IHC ;  Rat.  222
111 [IF=3.643] Wedn AM et al. Time and sex dependency of hemodynamic, renal, and survivability effects of endotoxemia in rats. Saudi Pharm J. 2020 Jan;28(1):127-135.  IHC-P ;  Rat.  222
111 [IF=3.508] Juin-Hong Cherng. et al. Hemostasis and Anti-Inflammatory Abilities of AuNPs-Coated Chitosan Dressing for Burn Wounds. J PERS MED. 2022 Jul;12(7):1089  IHC ;  Rat.  222
111 [IF=3.263] Mahmoud E. Youssef. et al. α7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996  IHC ;  Rat.  222
111 [IF=3.26] Zhao, Meng, et al. "The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection." International Journal of Molecular Sciences 18.6 (2017): 1229.  WB ;  Chicken.  222
111 [IF=3.222] Hager S. Zoghroban. et al. The impact of L-citrulline on murine intestinal cell integrity, immune response, and arginine metabolism in the face of Giardia lamblia infection. ACTA TROP. 2023 Jan;237:106748  IHC ;  Rat.  222
111 [IF=3.152] Dan-dan Wu. et al. STING mediates SU5416/hypoxia-induced pulmonary arterial hypertension in rats by regulating macrophage NLRP3 inflammasome activation. IMMUNOBIOLOGY. 2023 Mar;228:152345  IHC ;  Rat.  222
111 [IF=3.118] Liu J et al. Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus. Int Immunopharmacol. 2018 Nov;64:60-68.   WB ;  Chicken.  222
111 [IF=3.04] Tawfik KM et al. Neuroprotective mechanisms of sildenafil and selenium in PTZ-kindling model: Implications in epilepsy.Eur J Pharmacol. 2018 Aug 15;833:131-144.  IHC-P ;  Mouse.  222
111 [IF=2.82] Zhao et al. Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats. (2016) J.Histochem.Cytochem. 64:125-37  WB ;  Rat.  222
111 [IF=2.78] Li et al. Autologous transplantation of adipose-derived mesenchymal stem cells attenuates cerebral ischemia and reperfusion injury through suppressing apoptosis and inducible nitric oxide synthase. (2012) Int.J.Mol.Med. 29:848-54  IHC ;  Rat.  222
111 [IF=2.76] Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017).  WB ;  Rat.  222
111 [IF=2.741] Shao,et al.Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken.(2018) Environmental Science and Pollution Research. 25:7719-7729.  WB + IF ;  Chicken.  222
111 [IF=2.6] Wu TH et al. The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. J Oncol. 2019 Nov 3;2019:9206951.  IHF-P ;  Mouse.  222
111 [IF=2.431] Yang X et al. The Activation of Heat-Shock Protein After Copper (II) and/or Arsenic (III)-Induced Imbalance of Homeostasis, Inflammatory Response in Chicken Rectum. Biol Trace Elem Res. 2019 Aug 31.  WB ;  Cockerel.  222
111 [IF=2.342] Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. Medchemcomm. 2018 Jul 19;9(9):1502-1510.  IHC-P ;  Mouse.  222
111 [IF=2.342] Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.  IHC-P ;  Mouse.  222
111 [IF=1.931] Zhao et al. Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway. (2016) Evid.Based.Complement.Alternat.Med. 2016:8219287  WB/IHC ;  Rat.  222
111 [IF=1.858] Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 Aug;:  WB ;  Sheep.  222
111 [IF=1.832] Saghir SAM et al. Thymoquinone-PLGA-PVA Nanoparticles Ameliorate Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulation of Inflammatory Cytokines and iNOS Signaling. Animals (Basel). 2019 Nov 11;9(11). pii: E951.  IHC-P ;  Rat.  222
研究领域 细胞生物  免疫学  神经生物学  信号转导  生长因子和激素  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Rat, Chicken,  (predicted: Human, Mouse, Dog, Rabbit, Guinea Pig, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 130 kDa
检测分子量
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse NOS-2: 14-100/1144 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Function:
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Subunit:
Homodimer. Binds SLC9A3R1.

Subcellular Location:
Cytosol

Tissue Specificity:
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Similarity:
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.

SWISS:
P29477

Gene ID:
18126

Database links:

Entrez Gene: 4843 Human

Entrez Gene: 18126 Mouse

Entrez Gene: 24599 Rat

Omim: 163730 Human

SwissProt: P35228 Human

SwissProt: P29477 Mouse

SwissProt: Q06518 Rat

Unigene: 709191 Human

Unigene: 2893 Mouse

Unigene: 10400 Rat



合成与降解（Synthesis and Degradation）
催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3)。
产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (bs-2072R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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