| 产品编号 | bs-3301R |
| 英文名称 | Phospho-NMDAR1 (Ser890) Rabbit pAb |
| 中文名称 | 磷酸化离子型谷氨酸受体1抗体 |
| 别 名 | NMDAR1(phospho S890); p-NMDAR1(phospho S890); NMDA-NR1; N-Methyl-d-Asprtate receptor 1; GRIN1; NMDA1; NR1; Glutamate [NMDA] receptor subunit zeta 1; Glutamate receptor ionotropic N methyl D aspartate 1; Grin 1; Grin1; N methyl D aspartate receptor channel; N-methyl-D-aspartate receptor; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; NMDA 1; NMDA NR1; NMDA R1; NMDA receptor 1; NMDA1; NMDAR 1; NMDAR; NR 1; NMDZ1_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Rat, (predicted: Mouse, Chicken, Dog, Cow, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 103 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human NMDAR1 around the phosphorylation site of Ser890: AS(p-S)F <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Subunit: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity). Interacts with LRFN1 and LRFN2 (By similarity). Interacts with MYZAP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Post-translational modifications: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. DISEASE: Defects in GRIN1 are the cause of mental retardation autosomal dominant type 8 (MRD8) [MIM:614254]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. SWISS: Q05586 Gene ID: 2902 Database links: Entrez Gene: 2902 Human Entrez Gene: 14810 Mouse Omim: 138249 Human SwissProt: Q05586 Human SwissProt: P35438 Mouse Unigene: 558334 Human Unigene: 278672 Mouse Unigene: 9840 Rat 神经细胞标志物 (NMDAR1)N-甲基-D-天门冬氨酸受体(NMDAR)是兴奋性氨基酸受体亚型之一，是由NMDAR1与不同的NMDAR2亚基组成的异聚体。 NMDAR1又称GluR1 (Glutamate Receptor 1)近年实验研究发现，许多NMDAR拮抗药均具有镇痛活性，表明NMDAR在痛觉传递中具有重要作用，这为新型镇痛药的研究开发提供了新的作用靶点。 |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-NMDAR1(Ser890) Polyclonal Antibody, Unconjugated(bs-3301R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-NMDAR1(Ser890) Polyclonal Antibody, Unconjugated(bs-3301R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
