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RabbitAnti-Phospho-PERK (Thr980) Rabbit pAb antibody (bs-3330R)
~~~促销，代码KXJ230206~~~

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说明书： 50ul 100ul 200ul

50ul/1180.00元

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100ul/1980.00元

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200ul/2800.00元

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产品编号	bs-3330R
英文名称	Phospho-PERK (Thr980) Rabbit pAb
中文名称	磷酸化蛋白激酶样内质网激酶抗体
别名	p-PERK(Thr980); PERK(phospho Thr980); PERK(phospho T980); mo(Thr980)/hu (Thr982)/rat(Thr974); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase.
	Specific References (64) \| bs-3330R has been referenced in 64 publications. 111 [IF=41.845] Ashley R. Helseth. et al. Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice. Science. 2021 Apr;372(6540): IHC ; Mouse. 222 PubMed:33888613 111 [IF=25.606] Raines, Lydia N.. et al. PERK is a critical metabolic hub for immunosuppressive function in macrophages. Nat Immunol. 2022 Feb;23(3):431-445 FC,WB ; Mouse. 222 PubMed:10.1038/s41590-022-01145-x 111 [IF=9.988] Yue Zhang. et al. Endoplasmic reticulum stress-controlled autophagic pathway promotes polystyrene microplastics-induced myocardial dysplasia in birds. ENVIRON POLLUT. 2022 Oct;311:119963 WB ; Chicken. 222 PubMed:35973452 111 [IF=9.462] Santanam et al. Atg7 cooperates with Pten loss to drive prostate cancer tumor growth. (2016) Genes.De. 30:399-407 WB ; Mouse. 222 PubMed:26883359 111 [IF=9.429] Wang, Daquan. et al. Protection of zero-valent iron nanoparticles against sepsis and septic heart failure. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22 WB ; Mouse. 222 PubMed:36064371 111 [IF=8.701] Dandan Wu. et al. Unfolded Protein Response Factor ATF6 Augments T Helper Cell Responses and Promotes Mixed Granulocytic Airway Inflammation. MUCOSAL IMMUNOL. 2023 May;: WB ; Mouse. 222 PubMed:37209959 111 [IF=7.376] Meng Sun. et al. Silibinin protects against sepsis and septic myocardial injury in an NR1H3-dependent pathway. FREE RADICAL BIO MED. 2022 Jul;187:141 WB ; Mouse. 222 PubMed:35640818 111 [IF=7.376] Fang Dong. et al. Trimethylamine N-oxide promotes hyperoxaluria-induced calcium oxalate deposition and kidney injury by activating autophagy. Free Radical Bio Med. 2021 Nov;: WB ; Mouse,Human. 222 PubMed:34767921 111 [IF=6.706] Yujie Zhong. et al. Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats. NUTRIENTS. 2022 Jan;14(23):4994 WB, IHC ; Rat. 222 PubMed:36501024 111 [IF=6.706] Yujie Zhong. et al. Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy. NUTRIENTS. 2023 Jan;15(9):2164 WB,IHC ; Rat,Human. 222 PubMed:10.3390/nu15092164 111 [IF=6.543] Fan Kexia. et al. Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Oxid Med Cell Longev. 2022;2022:5652586 WB ; Mouse. 222 PubMed:35368865 111 [IF=6.284] Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02 WB ; Rat. 222 PubMed:33651226 111 [IF=6.268] Nadya Al-Yacoub. et al. Mutation in FBXO32 causes dilated cardiomyopathy through up-regulation of ER-stress mediated apoptosis. Commun Biol. 2021 Jul;4(1):1-12 WB ; Human. 222 PubMed:34272480 111 [IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242 WB ; Pig. 222 PubMed:37108405 111 [IF=6.1] Yiming Wang. et al. Exosome modification to better alleviates endoplasmic reticulum stress induced chondrocyte apoptosis and osteoarthritis. BIOCHEM PHARMACOL. 2022 Nov;:115343 WB ; Human. 222 PubMed:36370754 111 [IF=6.081] Ji-Eun Kim. et al. AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats. Biomedicines. 2021 Aug;9(8):1069 WB ; Rat. 222 PubMed:34440273 111 [IF=6.011] Ravishankar Chandrasekaran. et al. Mitoquinone mesylate attenuates pathologic features of lean and obese allergic asthma in mice. AM J PHYSIOL-LUNG C. 2022 Dec;(Articles in Press): WB ; Mouse. 222 PubMed:36511516 111 [IF=5.924] Kuan-Ming Lai. et al. Crassolide Induces G2/M Cell Cycle Arrest, Apoptosis, and Autophagy in Human Lung Cancer Cells via ROS-Mediated ER Stress Pathways. INT J MOL SCI. 2022 Jan;23(10):5624 WB ; Human. 222 PubMed:35628435 111 [IF=5.834] Jinlong Tan. et al. Emerging evidence for poxvirus-mediated unfolded protein response: Lumpy skin disease virus maintains self-replication by activating PERK and IRE1 signaling. FASEB J. 2023 Apr;37(5):e22902 WB ; Bovine. 222 PubMed:37014316 111 [IF=5.778] Yuting Wang. et al. Polybrominated diphenyl ethers quinone-induced intracellular protein oxidative damage triggers ubiquitin-proteasome and autophagy-lysosomal system activation in LO2 cells. Chemosphere. 2021 Jul;275:130034 WB ; Human. 222 PubMed:33652285 111 [IF=5.717] Jianlong Du. et al. FXR, a Key Regulator of Lipid Metabolism, Is Inhibited by ER Stress-Mediated Activation of JNK and p38 MAPK in Large Yellow Croakers (Larimichthys crocea) Fed High Fat Diets. Nutrients. 2021 Dec;13(12):4343 WB ; Fish. 222 PubMed:34959897 111 [IF=5.656] Kim JE et al. TRPC6-Mediated ERK1/2 Activation Increases Dentate Granule Cell Resistance to Status Epilepticus Via Regulating Lon Protease-1 Expression and Mitochondrial Dynamics. Cells. 2019 Nov 1;8(11). pii: E1376. WB ; Rat. 222 PubMed:31683954 111 [IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551 WB,IF,IHC ; Mouse. 222 PubMed:32996162 111 [IF=5.27] Kucuksayan, Ertan, et al. "Neutral Sphingomyelinase Inhibition Decreases ER Stress-Mediated Apoptosis and Inducible Nitric Oxide Synthase in Retinal Pigment Epithelial Cells." Free Radical Biology and Medicine (2014). WB ; Human. 222 PubMed:24742815 111 [IF=5.194] Li Wang. et al. New sight into interaction between endoplasmic reticulum stress and autophagy induced by vanadium in duck renal tubule epithelial cells. CHEM-BIOL INTERACT. 2022 May;:109981 WB ; Duck. 222 PubMed:35588787 111 [IF=5.118] I-Ta Lu. et al. (−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma. Mar Drugs. 2022 Feb;20(2):109 WB ; Human. 222 PubMed:10.3390/md20020109 111 [IF=5.1] Gerbino, Andrea, et al. "Functional Characterization of a Novel Truncating Mutation in Lamin A/C Gene in a Family with a Severe Cardiomyopathy with Conduction Defects." Cellular Physiology and Biochemistry 44.4 (2017): 1559-1577. WB ; Human. 222 PubMed:29197877 111 [IF=5.014] Ji-Eun Kim. et al. Epigallocatechin-3-Gallate and PEDF 335 Peptide, 67LR Activators, Attenuate Vasogenic Edema, and Astroglial Degeneration Following Status Epilepticus. Antioxidants-Basel. 2020 Sep;9(9):854 WB,IHC ; Rat. 222 PubMed:32933011 111 [IF=4.96] Wei Shi. et al. Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway. Biochem Pharmacol. 2021 Aug;190:114637 WB ; Human. 222 PubMed:34062127 111 [IF=4.94] Carmosino, Monica, et al. "The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca2+ handling." Journal of Cellular and Molecular Medicine (2016). WB, IF(ICC) ; Human. 222 PubMed:27421120
产品类型	磷酸化抗体
研究领域	肿瘤免疫学信号转导细胞凋亡转录调节因子激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, )
产品应用	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	119 kDa
检测分子量
细胞定位	细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthesised phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980: H(p-T)GQ
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010]. Function: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. Subunit: Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Tissue Specificity: Ubiquitous. Post-translational modifications: Autophosphorylated. N-glycosylated. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 1 protein kinase domain. SWISS: Q9Z2B5 Gene ID: 13666 Database links: Entrez Gene: 9451 Human Entrez Gene: 13666 Mouse Entrez Gene: 29702 Rat Omim: 604032 Human SwissProt: Q9NZJ5 Human SwissProt: Q9Z2B5 Mouse SwissProt: Q9Z1Z1 Rat Unigene: 591589 Human Unigene: 247167 Mouse Unigene: 24897 Rat
产品图片	Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human MCF-7-Tg (1 μM thapsigargin, 20 min) cell lysates Lane 3: Human MCF-7-phosphatase cell lysates Primary: Anti-Phospho-PERK (Thr980) (bs-3330R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30m Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-PERK (Thr980) antibody (bs-3330R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange lin Blank control (Black line): A431 (Black). Primary Antibody (green line): Rabbit Anti-PERK(Thr980) antibody (bs-3330R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rab Blank control: A431. Primary Antibody (green line): Rabbit Anti-PERK(Thr980) antibody (bs-3330R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Pro

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记