| 产品编号 | bs-3655R |
| 英文名称 | PP14 Rabbit pAb |
| 中文名称 | 胎盘蛋白14抗体 |
| 别 名 | Placental protein 14; alpha uterine protein; Pregnancy-associated endometrial alpha-2 globulin; GD; GdA; GdF; GdS; Glycodelin; Glycodelin F; Glycodelin S; PAEG; PAEP; PAEP protein; PEG; PEP; PP14; Pregnancy associated endometrial alpha 2 globulin; Progestagen associated endometrial protein(placental protein 14); Progestagen associated endometrial protein; Progesterone associated endometrial protein; MGC138509; MGC142288; PAEP_HUMAN. |
| 研究领域 | 肿瘤 免疫学 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glycodelin A/PP14: 101-180/180 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. A number of alternatively spliced transcript variants have been observed at this locus, but the full-length nature of only two, each encoding the same protein, has been determined. [provided by RefSeq, Jul 2008]. Function: This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. Subunit: Homodimer. Subcellular Location: Secreted. Post-translational modifications: At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: P09466 Gene ID: 5047 Database links: Entrez Gene: 5047 Human Omim: 173310 Human SwissProt: P09466 Human Unigene: 532325 Human 胎盘蛋白质14又称黄体激素子宫内膜癌相关蛋白。 胎盘蛋白质14是胎盘球蛋白的一种，该蛋白是子宫内膜分泌较高的黄体激素，目前多用于子宫内膜癌的研究 |
| 产品图片 |
Sample:
Lane 1: Human K562 cell lysates
Lane 2: K562 treated with 2ng/ml PMA for 2 days
Primary: Anti-PP14 (bs-3655R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
