| 产品编号 | bs-3876R |
| 英文名称 | APOA4 Rabbit pAb |
| 中文名称 | 载脂蛋白A4抗体 |
| 别 名 | Apo AIV; APOA 4; Apolipoprotein A IV; MGC142154; MGC142156. |
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Specific References (1) | bs-3876R has been referenced in 1 publications.
111
[IF=5.008] Wang et al. Proteome-based identification of apolipoprotein A-IV as an early diagnostic biomarker in liver fibrosis. (2017) Oncotarge. 8:88951-88964 WB ; Rat. 222
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| 研究领域 | 心血管 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Rat, (predicted: Mouse, Dog, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APOA4: 325-375/396 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties Function: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Subcellular Location: Secreted. Tissue Specificity: Synthesized primarily in the intestine and secreted in plasma. Post-translational modifications: Phosphorylation sites are present in the extracellular medium. Similarity: Belongs to the apolipoprotein A1/A4/E family. SWISS: P06727 Gene ID: 337 Database links: Entrez Gene: 337 Human Omim: 107690 Human SwissProt: P06727 Human Unigene: 1247 Human |
| 产品图片 |
Sample:
Brain (Rat) Lysate at 30 ug
Kidney (Rat) Lysate at 30 ug
Primary: Anti-APOA4 (bs-3876R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
