| 产品编号 | bs-4026R |
| 英文名称 | ALDOB Rabbit pAb |
| 中文名称 | 醛缩酶2抗体 |
| 别 名 | ALDB; ALDO2; Aldo-2; LIV10; ALDOB_HUMAN; ALDOB; Liver-type aldolase; 4.1.2.13; ALDOB_MOUSE; Aldolase 2; ALDOB_RAT; |
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Specific References (1) | bs-4026R has been referenced in 1 publications.
111
[IF=3.345] Xu et al. Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells. (2015) Mar.Drug. 13:1798-818 WB ; Mouse. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | 39 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALDOB: 261-364/364 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ALDOB is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Defects in ALDOB cause hereditary fructose intolerance. Subunit: Homotetramer. DISEASE: Hereditary fructose intolerance (HFI) [MIM:229600]: Autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the class I fructose-bisphosphate aldolase family. SWISS: P05062 Gene ID: 229 Database links: Entrez Gene: 229 Human Entrez Gene: 230163 Mouse Omim: 612724 Human SwissProt: P05062 Human SwissProt: Q91Y97 Mouse Unigene: 530274 Human Unigene: 482116 Mouse Unigene: 98207 Rat 醛缩酶（ALD）与机体能量代谢密切相关。醛缩酶含量比较丰富，分布也叫广泛，醛缩酶B是一种与糖酵解有关的酶类，在人类和哺乳动物组织中存在3种醛缩酶同工酶，即A(肌型)，B(肝型)和C（脑型）。其中B型基因定位于q13-q32，属胞浆酶。研究表明，ALDOB在肝细胞癌发展过程中呈现出一定规律的变化，与原发性肝癌有关。ALDOB蛋白主要定位于胞浆。 |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human SH-SY5Y cell lysates
Primary: Anti-ALDOB (bs-4026R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ALDOB) polyclonal Antibody, Unconjugated (bs-4026R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
