| 产品编号 | bs-5033R |
| 英文名称 | AGPAT4 Rabbit pAb |
| 中文名称 | 溶血磷脂酸酰基转移酶D抗体 |
| 别 名 | 1 acyl sn glycerol 3 phosphate acyltransferase delta; 1 acylglycerol 3 phosphate O acyltransferase 4; 1 AGP acyltransferase 4; 1 AGPAT4; 1-acyl-sn-glycerol-3-phosphate acyltransferase delta; 1-acylglycerol-3-phosphate O-acyltransferase 4(lysophosphatidic |
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Specific References (1) | bs-5033R has been referenced in 1 publications.
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[IF=0] Bradley, Ryan M., et al. "Data on acylglycerophosphate acyltransferase 4 (AGPAT4) during murine embryogenesis and in embryo-derived cultured primary neurons and glia." Data in Brief (2015). Mouse. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Agpat4: 231-330/378 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Agpat4 converts lysophosphatidic acid into phosphatidic acid by incorporating acyl moiety at the 2 position. The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. Function: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Subcellular Location: Membrane; Multi-pass membrane protein (Potential). Similarity: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. SWISS: Q9NRZ5 Gene ID: 56895 Database links: Entrez Gene: 56895 Human Entrez Gene: 68262 Mouse SwissProt: Q9NRZ5 Human SwissProt: Q8K4X7 Mouse Unigene: 353175 Human Unigene: 258300 Mouse Unigene: 41595 Rat |
| 产品图片 |
Sample:
Brain (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti-AGPAT4 (bs-5033R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 44 kD
Observed band size: 41 kD
Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-AGPAT4 Polyclonal Antibody, Unconjugated(bs-5033R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
