| 产品编号 | bs-5041R |
| 英文名称 | BDH1 Rabbit pAb |
| 中文名称 | 3-羟丁酸脱氢酶抗体 |
| 别 名 | 3 hydroxybutyrate dehydrogenase(heart, mitochondrial); BDH; BDH-1; D beta hydroxybutyrate dehydrogenase, mitochondrial; 3-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; mitochondrial; BDH_HUMAN . |
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Specific References (2) | bs-5041R has been referenced in 2 publications.
111
[IF=4.225]
111
[IF=4.225] Li Huihui. et al. Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α. Front Pharmacol. 2020 Aug;11:1255 WB ; Rat. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 转运蛋白 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BDH1: 241-343/343 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. Subunit: Homotetramer. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. SWISS: Q02338 Gene ID: 622 Database links: Entrez Gene: 622 Human Entrez Gene: 71911 Mouse Omim: 603063 Human SwissProt: Q02338 Human SwissProt: Q80XN0 Mouse Unigene: 274539 Human Unigene: 293470 Mouse |
| 产品图片 |
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-BDH1 antibody (bs-5041R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min atroom temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
