| 产品编号 | bs-4057R |
| 英文名称 | DEGS1 Rabbit pAb |
| 中文名称 | 退行性精母细胞同源物1抗体 |
| 别 名 | DES1; DEGS; DEGS-1; Des-1; FADS7; HLD18; MIG15; MLD; Mdes; DEGS1_BOVIN; DEGS1; Degenerative spermatocyte homolog 1; Dihydroceramide desaturase-1; Retinol isomerase; 1.14.19.17; DEGS1_HUMAN; Cell migration-inducing gene 15 protein; Membrane lipid desaturas |
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Specific References (2) | bs-4057R has been referenced in 2 publications.
111
[IF=4.36] Meixia Zhu. et al. Danhe granule ameliorates nonalcoholic steatohepatitis and fibrosis in rats by inhibiting ceramide de novo synthesis related to CerS6 and CerK. J ETHNOPHARMACOL. 2022 May;:115427 WB ; Rat. 222
111
[IF=0.762] Inoue C et al. Vaticanol C, a phytoalexin, induces apoptosis of leukemia and cancer cells by modulating expression of multiple sphingolipid metabolic enzymes. Nagoya J Med Sci
. 2020 May;82(2):261-280. WB ; Human. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | 38 |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DEGS1: 21-120/323 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Function: Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Subcellular Location: Mitochondrion. Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitous. Post-translational modifications: Myristoylation can target the enzyme to the mitochondria leading to an increase in ceramide levels. Similarity: Belongs to the fatty acid desaturase family. DEGS subfamily. SWISS: O15121 Gene ID: 8560 Database links: Entrez Gene: 8560 Human Entrez Gene: 13244 Mouse Entrez Gene: 100192315 Sheep SwissProt: O15121 Human SwissProt: O09005 Mouse |
| 产品图片 |
Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human 293T cell lysates
Primary: Anti-DEGS1 (bs-4057R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 38 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
