| 产品编号 | bs-5131R |
| 英文名称 | GLO1 Rabbit pAb |
| 中文名称 | 乙二醛酶1抗体 |
| 别 名 | Aldoketomutase; GLOD1; Glx I; GLYI; Glyoxalase I; Ketone aldehyde mutase; Lactoyl glutathione lyase; S D lactoylglutathione methylglyoxal lyase; LGUL_HUMAN; glo1; glyoxalase domain containing 1; Ketone-aldehyde mutase; Methylglyoxalase. |
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Specific References (1) | bs-5131R has been referenced in 1 publications.
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[IF=4.49] Seyeon Oha et al. The attenuating effects of pyridoxamine on adipocyte hypertrophy and inflammation differ by adipocyte location. The Journal of Nutritional Biochemistry.2019 October, 72: 108173. WB&IHF ; Rat. 222
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| 研究领域 | 肿瘤 免疫学 糖尿病 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLO1: 101-184/184 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The enzyme encoded by GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. Subunit: Homodimer. Similarity: Belongs to the glyoxalase I family. SWISS: Q04760 Gene ID: 2739 Database links: Entrez Gene: 2739 Human Entrez Gene: 109801 Mouse Omim: 138750 Human SwissProt: Q04760 Human SwissProt: Q9CPU0 Mouse Unigene: 268849 Human Unigene: 261984 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLO1) Polyclonal Antibody, Unconjugated (bs-5131R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
