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RabbitAnti-phospho-APC1 (Ser355) Rabbit pAb  antibody (bs-5173R)
~~~促销,代码KXJ230206~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-5173R
英文名称 phospho-APC1 (Ser355) Rabbit pAb
中文名称 磷酸化细胞周期末期促进复合蛋白APC1抗体
别    名 Apc1(phospho S355); phospho-ANAPC1(Ser355); ANAPC 1; ANAPC1; Anaphase promoting complex subunit 1; anaphase-promoting complex 1(meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; Apc 1; APC1; APC1_HUMAN; Cyclosome subunit 1; MCPR; Meiotic checkpoint regulator; Mitotic checkpoint regulator; Protein Tsg 24; Protein Tsg24; Testis-specific gene 24 protein; TSG 24; TSG24.  
产品类型 磷酸化抗体 
研究领域 细胞生物  免疫学  细胞周期蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 212 kDa
检测分子量
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser355: AH(p-S)PA 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011].

Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Subunit:
The APC/C is composed of at least 12 subunits.

Post-translational modifications:
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.

Similarity:
Belongs to the APC1 family.
Contains 4 PC repeats.

SWISS:
Q9H1A4

Gene ID:
64682

Database links:

Entrez Gene: 64682 Human

Entrez Gene: 535565 Cow

Entrez Gene: 475750 Dog

Entrez Gene: 17222 Mouse

Omim: 608473 Human

SwissProt: E1BAB6 Cow

SwissProt: E2RLM2 Dog

SwissProt: Q9H1A4 Human



产品图片
Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti- phospho-APC1 (Ser355) (bs-5173R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 212 kD Observed band size: 212 kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-APC1 (Ser355)) Polyclonal Antibody, Unconjugated (bs-5173R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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