| 产品编号 | bs-5384R |
| 英文名称 | phospho-HMG14 (Ser20+Ser24) Rabbit pAb |
| 中文名称 | 磷酸化高迁移率核小体结合蛋白1 |
| 别 名 | HMG14(phospho Ser20+Ser24); HMG14(phospho S20+S24); High mobility group(nonhistone chromosomal) protein 14; High mobility group nucleosome binding 1; High mobility group nucleosome binding domain containing protein 1; High mobility group protein 14; High-mobility group nucleosome binding domain 1; HMG14; HMGN 1; HMGN1; MGC104230; MGC117425; Nonhistone chromosomal protein HMG-14; Nonhistone chromosomal protein HMG14; FLJ27265; FLJ31471. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 神经生物学 信号转导 细胞周期蛋白 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human HMG14 around the phosphorylation site of Ser20+Ser24: RR(p-S)ARL(p-S)AK |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Chromosomal protein HMG14 binds to the inner side of the nucleosomal DNA, thus altering the interaction between the DNA and the histone octamer and is involved in the process that maintains transcribable genes in an appropriate chromatin conformation. HMG14 has been implicated as a contributing factor in the etiology of Down syndrome. Function: Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2. Subcellular Location: Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus. Post-translational modifications: Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization. Similarity: Belongs to the HMGN family. SWISS: P05114 Gene ID: 3150 Database links: Entrez Gene: 3150 Human Entrez Gene: 100044391 Mouse Entrez Gene: 15312 Mouse Omim: 163920 Human SwissProt: P05114 Human SwissProt: P18608 Mouse Unigene: 87121 Cow Unigene: 356285 Human Unigene: 702472 Human Unigene: 2756 Mouse HMGN1是一个核仁结合蛋白, 属于核小体结合高速迁移蛋白家族(A family of High Mobility Group Proteins that bind to Nucleosomesn, HMGN)含量蛋白较高,能稳定染色质的二级结构, 修复紫外线诱导的染色质DNA损伤. HMGN1的缺失可导致细胞增殖和生长发生紊乱, 通过打乱细胞的生长控制机制使一些致癌因素更为敏感并发生恶性转化. 研究表明, HMGN1在细胞核内是动态表达的,与细胞的新陈代谢有关. HMGN1蛋白和核仁的相互作用可改变染色质的结构, 并且能调节各种与DNA相关的包括转录的细胞核作用. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
