| 产品编号 | bs-5468R |
| 英文名称 | phospho-MAP4 (Ser1073) Rabbit pAb |
| 中文名称 | 磷酸化微管相关蛋白4抗体 |
| 别 名 | MAP4(phospho Ser1073); MAP4(phospho S1073); DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞周期蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 121 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MAP4 around the phosphorylation site of Ser1073: VG(p-S)LD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the C-terminus negatively regulates MAP4 activity to promote microtubule assembly. Function: Non-neuronal microtubule-associated protein. Promotes microtubule assembly. Subunit: Interacts with SEPT2; this interaction impedes tubulin-binding. Subcellular Location: Cytoplasm, cytoskeleton. Post-translational modifications: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338. Similarity: Contains 4 Tau/MAP repeats. SWISS: P27816 Gene ID: 4134 Database links: Entrez Gene: 4134 Human Entrez Gene: 17758 Mouse Omim: 157132 Human SwissProt: P27816 Human SwissProt: P27546 Mouse Unigene: 517949 Human Unigene: 217318 Mouse Unigene: 443428 Mouse Unigene: 484451 Mouse Unigene: 203122 Rat |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MAP4 (Ser1073) (bs-5468R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
