| 产品编号 | bs-4193R |
| 英文名称 | CBS Rabbit pAb |
| 中文名称 | 丝氨酸羧甲半胱氨酸合成酶抗体 |
| 别 名 | CBSL; HIP4; CBS_HUMAN; CBS; Beta-thionase; Serine sulfhydrase; 4.2.1.22; CBS_MOUSE; CBS_RAT; Hemoprotein H-450; cystathionine beta-synthase; cystathionine-beta-synthase |
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Specific References (2) | bs-4193R has been referenced in 2 publications.
111
[IF=6.543] Yuan Jiyan. et al. Vitamin B12 Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway. Oxid Med Cell Longev. 2021;2021:7936316 WB ; Mouse. 222
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[IF=2.53] Shiina, Takahiko, et al. "Inhibitory action of hydrogen sulfide on esophageal striated muscle motility in rats." European Journal of Pharmacology (2016). WB ; Rat. 222
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| 研究领域 | 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Chicken, Cow, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61kDa |
| 检测分子量 | 61 |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HIP4: 177-280/400 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2010]. SWISS: P35520 Gene ID: 875 Database links: Entrez Gene: 875 Human Entrez Gene: 12411 Mouse Omim: 613381 Human SwissProt: P35520 Human SwissProt: Q91WT9 Mouse Unigene: 533013 Human |
| 产品图片 |
Sample:
Liver(Mouer) Cell Lysate at 40 ug
Primary: Anti-CBS (bs-4193R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CBS) Polyclonal Antibody, Unconjugated (bs-4193R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
