| 产品编号 | bs-4245R |
| 英文名称 | LONP1 Rabbit pAb |
| 中文名称 | 线粒体离子肽酶1抗体 |
| 别 名 | Serine protease 15; hLON ATP dependent protease; Lon protease like protein; LonHS; LONP; PIM1; PRSS15; hLON; LON; lon peptidase 1, mitochondrial; LON protease; Mitochondrial ATP dependent protease Lon; Mitochondrial Ion peptidase 1; Protease serine 15. |
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Specific References (4) | bs-4245R has been referenced in 4 publications.
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[IF=9.207] Muñoz Vitor Rosetto. et al. Effects of short-term endurance and strength exercise in the molecular regulation of skeletal muscle in hyperinsulinemic and hyperglycemic Slc2a4+/− mice. CELL MOL LIFE SCI. 2023 May;80(5):1-16 WB ; Mouse. 222
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[IF=8.44] Zai, Zhuoyan. et al. Estrogen antagonizes ASIC1a-induced chondrocyte mitochondrial stress in rheumatoid arthritis. J TRANSL MED. 2022 Dec;20(1):1-14 IF ; Rat. 222
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[IF=3.998] Renata R. Braga. et al. Exercise alters the mitochondrial proteostasis and induces the mitonuclear imbalance and UPR mt in the hypothalamus of mice. Sci Rep-Uk. 2021 Feb;11(1):1-13 WB ; Mouse. 222
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[IF=2.766] Nie et al. Down-regulating overexpressed human Lon in cervical cancer suppresses cell proliferation and bioenergetics. (2013) PLoS.One. 8:e81084 IHC ; Human. 222
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| 研究领域 | 肿瘤 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LONP1: 361-460/959 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
LONP1 encodes a mitochondrial matrix protein in the Lon family of ATP dependent proteases. It binds a specific sequence in the light and heavy chain promoters of the mitochondrial genome which are involved in regulation of DNA replication and transcription. Subunit: Homohexamer or homoheptamer. Organized in a ring with a central cavity. DNA and RNA binding is stimulated by substrate and inhibited by ATP binding. Interacts with PEO1 and mitochondrial DNA polymerase subunit POLG. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Duodenum, heart, lung and liver, but not thymus. Similarity: Belongs to the peptidase S16 family. Contains 1 Lon domain. SWISS: P36776 Gene ID: 9361 Database links: Entrez Gene: 9361 Human Entrez Gene: 74142 Mouse Omim: 605490 Human SwissProt: P36776 Human SwissProt: Q8CGK3 Mouse Unigene: 350265 Human Unigene: 329136 Mouse Unigene: 146805 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LONP1) Polyclonal Antibody, Unconjugated (bs-4245R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
