| 产品编号 | bs-5795R |
| 英文名称 | Robo4 Rabbit pAb |
| 中文名称 | 肿瘤血管内皮调节蛋白Robo4抗体 |
| 别 名 | Magic roundabout; MRB; Robo 4; Roundabout homolog 4; Roundabout homolog 4 magic roundabout; Roundabout homolog 4 Precursor; ROBO4_HUMAN. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 108 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Robo4: 261-360/1007 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Robo4 is a vascular specific receptor that is a repulsive cue to migrating endothelial cells during vascular development. Guidance and patterning of axons are orchestrated by cell surface receptors and ligands that provide directional cues. Interactions between the Robo receptor and Slit ligand families of proteins initiate signaling cascades that repel axonal outgrowth. In contrast to known neuronal Robo family members, the arrangement of the extracellular domains of Robo4 diverges significantly from that of all other Robo family members. Robo4 is specifically expressed in the vascular endothelium during murine embryonic development; the only Robo family member expressed in primary endothelial cells. Robo4 also has a role in regulating the migration of endothelial cells during tumor angiogenesis. Function: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit protein. Subunit: Interacts with SLIT2 and ENAH. Tissue Specificity: Specifically expressed in endothelial cells. Expressed at sites of angiogenesis in different tumor types. Similarity: Belongs to the immunoglobulin superfamily. ROBO family. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Gene ID: 54538 Database links:
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| 产品图片 |
Sample:
Lane 1: Recombinant human ROBO4 protein, C-His (HEK293)(bs-43625P)
Primary: Anti-Robo4 (bs-5795R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 60 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
