| 产品编号 | bs-5722R |
| 英文名称 | RASL10A Rabbit pAb |
| 中文名称 | Ras样蛋白家族10A抗体 |
| 别 名 | RRP22; 2210403B10Rik; RGD1306100; RSLAA_HUMAN; RASL10A; Ras-like protein RRP22; Ras-related protein on chromosome 22; 3.6.5.2; RSLAA_MOUSE; Ras-related protein on chromosome 22 homolog; RAS like family 10 member A; RAS-like, family 10, member A; RAS-like family 10 member A |
| 研究领域 | 肿瘤 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RASL10A: 51-150/203 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Ras proteins are members of a superfamily of related small GTPases. RRP22 can be posttranslationally modified by farnesyl. RRP22 inhibits cell growth and promotes caspase-independent cell death. The specific function of RASL10A is not yet known. Function: Potent inhibitor of cellular proliferation. Subunit: Interacts with UBF/UBTF. Subcellular Location: Cell membrane, Cytoplasmic side. Nucleus, nucleolus. Note=May cycle in and out of the nucleolus in a GTP-dependent manner. Tissue Specificity: Expression appears to be strictly limited to the central nervous system. Post-translational modifications: Isoprenylation is essential for nucleolar localization, and the proliferation-inhibiting activity of RASL10A. Similarity: Belongs to the small GTPase superfamily. Ras family. SWISS: Q92737 Gene ID: 10633 Database links: Entrez Gene: 10633 Human Entrez Gene: 75668 Mouse Omim: 602220 Human SwissProt: Q92737 Human SwissProt: Q8K5A4 Mouse Unigene: 73088 Human Unigene: 374836 Mouse Unigene: 50230 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RASL10A) Polyclonal Antibody, Unconjugated (bs-5722R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
