| 产品编号 | bs-5841R |
| 英文名称 | MGAT5 Rabbit pAb |
| 中文名称 | 糖蛋白GNTVA抗体 |
| 别 名 | 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; 6-N-acetylglucosaminyltransferase; alpha 1, 3(6) mannosylglycoprotein; alpha 1, 6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase; alpha mannoside beta 1, 6 N acetylglucosaminylt |
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Specific References (1) | bs-5841R has been referenced in 1 publications.
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[IF=3.375] Yang P et al. Adoptive transfer of polarized M2c macrophages ameliorates acute rejection in rat liver transplantation. Am J Transl Res. 2020 Jun 15;12(6):2614-2626. WB ; Rat. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞粘附分子 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Dog, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 85 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGAT5: 201-300/741 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MGAT5 is a mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies. Function: Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Similarity: Belongs to the glycosyltransferase 18 family. SWISS: Q09328 Gene ID: 4249 Database links: Entrez Gene: 4249 Human Entrez Gene: 107895 Mouse Omim: 601774 Human SwissProt: Q09328 Human SwissProt: Q8R4G6 Mouse Unigene: 651869 Human Unigene: 214682 Mouse Unigene: 391592 Mouse Unigene: 472488 Mouse Unigene: 53998 Rat |
| 产品图片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- MGAT5 (bs-5841R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MGAT5 ) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MGAT5 ) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
