| 产品编号 | bs-5888R |
| 英文名称 | Hyaluronidase2 Rabbit pAb |
| 中文名称 | 透明质酸酶2/玻璃酸酶2抗体 |
| 别 名 | Hyal 2; Hyal-2; Hyal2; HYAL2_HUMAN; Hyaluronidase 2; Hyaluronidase 2 Precursor; Hyaluronidase-2; Hyaluronoglucosaminidase 2; Hyaluronoglucosaminidase-2; LUCA 2; LUCA-2; LUCA2; Lung carcinoma protein 2; lysosomal hyaluronidase; PH 20 homolog. |
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Specific References (2) | bs-5888R has been referenced in 2 publications.
111
[IF=6.438] Anna Kocurkova. et al. Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development. BIOFACTORS. 2023 May;: IHC ; Mouse. 222
111
[IF=4.879] Petra Žádníková. et al. The Degradation of Hyaluronan in the Skin. Biomolecules. 2022 Feb;12(2):251 WB,IHC,IF ; Human. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HYAL2: 111-210/473 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. Function: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. Subunit: Interacts with MST1R. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Widely expressed. No expression detected in adult brain. Similarity: Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain. SWISS: Q12891 Gene ID: 8692 Database links: Entrez Gene: 8692 Human Entrez Gene: 15587 Mouse Omim: 603551 Human SwissProt: Q12891 Human SwissProt: O35632 Mouse Unigene: 76873 Human Unigene: 440982 Mouse Unigene: 4834 Mouse Unigene: 6149 Pig Unigene: 8278 Rat |
| 产品图片 |
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-Hyaluronidase2 (bs-5888R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hyluronidase 2 Polyclonal Antibody, Unconjugated(bs-5888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
