| 产品编号 | bs-5924R |
| 英文名称 | IMPDH2 Rabbit pAb |
| 中文名称 | 5'肌苷磷酸脱氢酶2抗体 |
| 别 名 | IMP(inosine monophosphate) dehydrogenase 2; IMP dehydrogenase 2; IMP oxireductase 2; IMPD 2; IMPD2; IMPDH 2; IMPDH II; IMPDH-II; Impdh2; IMPDHII; Inosine 5' monophosphate dehydrogenase 2; Inosine-5''-monophosphate dehydrogenase 2; IMDH2_HUMAN. |
| 研究领域 | 肿瘤 免疫学 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IMPDH2: 441-514/514 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. Function: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the IMPDH/GMPR family. Contains 2 CBS domains. SWISS: P12268 Gene ID: 3615 Database links: Entrez Gene: 3615 Human Entrez Gene: 23918 Mouse Omim: 146691 Human SwissProt: P12268 Human SwissProt: P24547 Mouse Unigene: 654400 Human Unigene: 6065 Mouse Unigene: 8093 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IMPDH2) Polyclonal Antibody, Unconjugated (bs-5924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IMPDH2) Polyclonal Antibody, Unconjugated (bs-5924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
