| 产品编号 | bs-5995R |
| 英文名称 | CAMTA1 Rabbit pAb |
| 中文名称 | 钙调蛋白结合转录激活因子1抗体 |
| 别 名 | Calmodulin-binding transcription activator 1; Calmodulin binding transcription activator 1; CMTA1; KIAA0833; CMTA1_HUMAN. |
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Specific References (1) | bs-5995R has been referenced in 1 publications.
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[IF=5.241] Pan, Ruijun. et al. CAMTA1–PPP3CA–NFATc4 multi-protein complex mediates the resistance of colorectal cancer to oxaliplatin. Cell Death Discov. 2022 Mar;8(1):1-13 IHC,WB ; Human. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 183 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CAMTA1: 101-200/1673 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CAMTA1 is a transcription activator which is a putatuove tumor suppressor. It is thought to interact with calmodulin. Defects in CAMTA1 are detected in patients with oligodendroglioma and astrocytoma. Function: Transcriptional activator. May act as a tumor suppressor. Subunit: May interact with calmodulin (Potential). Subcellular Location: Nucleus (Probable). Cytoplasm. Tissue Specificity: Normally expressed in non-neoplastic adult central nervous system tissues: detected in whole brain, cerebellum, brain cortex, occipital lobe, frontal lobe, temporal lobe, putamen. Expression levels are low in oligodendroglial tumors, and are reduced by half in oligodendroglioma and astrocytoma cases with 1p loss of heterozygosity. Detected in neuroblastic-type cultured neuroblastoma cells. Expressed in heart and kidney. Similarity: Belongs to the CAMTA family. Contains 3 ANK repeats. Contains 1 CG-1 DNA-binding domain. Contains 1 IPT/TIG domain. Contains 3 IQ domains. SWISS: Q9Y6Y1 Gene ID: 23261 Database links: Entrez Gene: 23261 Human Entrez Gene: 100072 Mouse Omim: 611501 Human SwissProt: Q9Y6Y1 Human SwissProt: A2A898 Mouse Unigene: 397705 Human Unigene: 671020 Human Unigene: 318846 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAMTA1) Polyclonal Antibody, Unconjugated (bs-5995R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAMTA1) Polyclonal Antibody, Unconjugated (bs-5995R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Image was kindly submitted by Dr. Luan Zhou at the University of Massachusetts Medical School. Frozen mouse retina stained with Rabbit Anti-CAMTA1 Polyclonal Antibody (bs-5995R)
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
