| 产品编号 | bs-6019R |
| 英文名称 | Aminoacylase 1 Rabbit pAb |
| 中文名称 | 氨基酰化酶1抗体 |
| 别 名 | ACY 1; ACY1; ACY1D; ACYLASE; EC 3.5.1.14; N acyl L amino acid amidohydrolase; ACY1_HUMAN. |
| 研究领域 | 免疫学 信号转导 通道蛋白 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat, (predicted: Human, Mouse, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ACY1/Aminoacylase 1: 201-300/408 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Aminoacylase 1 is a cytosolic, homodimeric, zinc binding enzyme that catalyzes the hydrolysis of acylated L amino acids to L amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase 1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc binding enzymes. Function: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Subunit: Homodimer. Interacts with SPHK1. Subcellular Location: Cytoplasm. Tissue Specificity: Expression is highest in kidney, strong in brain and weaker in placenta and spleen. DISEASE: Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Similarity: Belongs to the peptidase M20A family. SWISS: Q03154 Gene ID: 95 Database links: Entrez Gene: 95 Human Entrez Gene: 109652 Mouse Omim: 104620 Human SwissProt: Q03154 Human SwissProt: Q99JW2 Mouse Unigene: 334707 Human Unigene: 7165 Mouse Unigene: 3679 Rat |
| 产品图片 |
Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Small intestine (Rat) Lysate at 40 ug
Primary: Anti-Aminoacylase 1 (bs-6019R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
